{"id":"a959e288-a1f7-5e99-a847-bc4c62eaf189","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-tcell-external-gate","predicate":"supports_got1_impaired_proliferation","statement":"Removing extracellular aspartate selectively reduced proliferation of Got1-silenced mouse CD8 cells, whereas control cells better tolerated its removal and viability was unchanged.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"27faa031-f631-5abb-876e-df33dd21042e","mechanism_event_label":"Cells depended more on external supply when synthesis was impaired.","subject":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"ac8f2c41-68f2-50aa-8678-8f9dcf12872e","slug":"mouse-effector-t-cell-expansion","display_name":"Mouse effector CD8 T-cell expansion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"27faa031-f631-5abb-876e-df33dd21042e","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-tcell-external-gate-event","event_type":"observed_relationship","label":"Cells depended more on external supply when synthesis was impaired.","description":"Removing extracellular aspartate selectively reduced proliferation of Got1-silenced mouse CD8 cells, whereas control cells better tolerated its removal and viability was unchanged.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ac8f2c41-68f2-50aa-8678-8f9dcf12872e","slug":"mouse-effector-t-cell-expansion","display_name":"Mouse effector CD8 T-cell expansion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bd15dd76-31ee-5c52-9180-c11de03fa913","slug":"mouse-got1","display_name":"Mouse cytosolic aspartate aminotransferase / Got1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse CD8 culture; Got1 knockdown with/without medium aspartate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Conditional auxotrophy is not a demonstrated human dietary deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Cells depended more on external supply when synthesis was impaired.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"13C metabolite tracing reveals glutamine and acetate as critical in vivo fuels for CD8 T cells. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38809979/ · DOI 10.1126/sciadv.adj1431","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; 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