{"id":"a93a76e1-9bf6-5328-aa2a-abb5e0917fba","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-fibroblast-dose-response","predicate":"increases","statement":"A one-hour exposure at oxygen pressures between 106 and 300 kPa increased proliferation of both normal and diabetic fibroblasts, with a dose-dependent peak at 250 kPa for normal and 200 kPa for diabetic cells, and the effects were not due to hydrostatic pressure per se.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"1a650412-8f4c-5856-8ce2-5b10b9c46752","mechanism_event_label":"There is a best pressure, and going higher does not help more.","subject":{"id":"581fa746-cb84-571b-87a0-4d461263f660","slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"},"object":{"id":"69d6c1bd-e95e-57e3-90b7-12312892851f","slug":"fibroblast-proliferation","display_name":"Human fibroblast proliferation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1a650412-8f4c-5856-8ce2-5b10b9c46752","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-fibroblast-dose-response-event","event_type":"biochemical_relationship","label":"There is a best pressure, and going higher does not help more.","description":"A one-hour exposure at oxygen pressures between 106 and 300 kPa increased proliferation of both normal and diabetic fibroblasts, with a dose-dependent peak at 250 kPa for normal and 200 kPa for diabetic cells, and the effects were not due to hydrostatic pressure per se.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"76025c48-83c1-5f3b-95cc-9545d94b98b3","slug":"fibroblast","display_name":"Dermal fibroblast","entity_type_key":"cell_type"},"role":"affected_cell","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"31bd23dc-1798-52f9-b1fe-d364807d184c","slug":"chamber-pressure-alone","display_name":"Raised chamber pressure without raised oxygen fraction","entity_type_key":"chemical_species"},"role":"excluded_factor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"581fa746-cb84-571b-87a0-4d461263f660","slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"69d6c1bd-e95e-57e3-90b7-12312892851f","slug":"fibroblast-proliferation","display_name":"Human fibroblast proliferation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/hbot-research/16984424.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"59b39998f7e669db88fb2c3e69315ddfd6a1c52c82ab06997527991ac456d65a\", \"start_char\": 0, \"end_char\": 1236, \"text_sha256\": \"59b39998f7e669db88fb2c3e69315ddfd6a1c52c82ab06997527991ac456d65a\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human fibroblasts from normal skin and from chronic diabetic foot ulcers","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"One hour of hyperbaric oxygen at 106 to 300 kPa, with a hydrostatic pressure control","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A dose-response in culture with a pressure control. The authors note the effects may not be due to oxygen availability alone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Hyperbaric oxygen research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.","comparator":null,"unit":null,"notes":"","entity":{"slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Human cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"There is a best pressure, and going higher does not help more.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hbot-p16984424] Dose-dependent hyperbaric oxygen stimulation of human fibroblast proliferation. 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The authors note the effects may not be due to oxygen availability alone.\nexposure: One hour of hyperbaric oxygen at 106 to 300 kPa, with a hydrostatic pressure control\nevidence_span: {\"source_cache\": \"artifacts/hbot-research/16984424.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"59b39998f7e669db88fb2c3e69315ddfd6a1c52c82ab06997527991ac456d65a\", \"start_char\": 0, \"end_char\": 1236, \"text_sha256\": \"59b39998f7e669db88fb2c3e69315ddfd6a1c52c82ab06997527991ac456d65a\"}\n[hbot-p16984424] Dose-dependent hyperbaric oxygen stimulation of human fibroblast proliferation. (1997). https://pubmed.ncbi.nlm.nih.gov/16984424/ DOI: 10.1046/j.1524-475x.1997.50206.x","model_system":"Human fibroblasts from normal skin and from chronic diabetic foot ulcers","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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