{"id":"a935dc86-919a-5cc3-9347-ae88c5ada76e","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-dc-il6","predicate":"reduces","statement":"DM7 reduced stimulus-dependent IL-6 release from human dendritic cells; at lower tested concentrations DM75 did not reproduce this effect.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c5f25c3b-9796-5a7e-ac34-a0607728b995","mechanism_event_label":"Preparation structure affected how much the cells released an inflammatory messenger.","subject":{"id":"d54fb89f-e04d-580a-aff2-a97bdab74c19","slug":"low-dm-lemon-pectin","display_name":"Low-methylesterified lemon pectin, DM7 preparation","entity_type_key":"chemical_species"},"object":{"id":"56602307-29c5-50e8-862e-87a94745e3ea","slug":"human-dc-il6-release","display_name":"Human dendritic-cell IL-6 release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c5f25c3b-9796-5a7e-ac34-a0607728b995","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-dc-il6-event","event_type":"biochemical_relationship","label":"Preparation structure affected how much the cells released an inflammatory messenger.","description":"DM7 reduced stimulus-dependent IL-6 release from human dendritic cells; at lower tested concentrations DM75 did not reproduce this effect.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d54fb89f-e04d-580a-aff2-a97bdab74c19","slug":"low-dm-lemon-pectin","display_name":"Low-methylesterified lemon pectin, DM7 preparation","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"56602307-29c5-50e8-862e-87a94745e3ea","slug":"human-dc-il6-release","display_name":"Human dendritic-cell IL-6 release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/pectin-research/29545800.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4b9e856ec2420c457b98f455ec243b742525b63d9382b69ef087456e0136cb42\", \"start_char\": 38875, \"end_char\": 39612, \"text_sha256\": \"8b75517328dfdbdd5a4290c5523e8136762b0456a8fd69af5e0e172edb168bda\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Receptor binding, reporter cells, human dendritic cells and mouse ileitis model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lemon pectin DM7 versus DM75; cell exposures 1-100 micrograms/mL; mice 3 mg/day before doxorubicin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Model-specific. Binding and cytokine experiments do not establish prevention of human inflammatory disease. Lack of increased measured SCFAs does not exclude every microbial contribution.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pectin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pectin","display_name":"Pectin, structurally heterogeneous plant polysaccharides","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Preparation structure affected how much the cells released an inflammatory messenger.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[pectin-p29545800] Dietary Fiber Pectin Directly Blocks Toll-Like Receptor 2-1 and Prevents Doxorubicin-Induced Ileitis. (2018). https://pubmed.ncbi.nlm.nih.gov/29545800/ DOI: 10.3389/fimmu.2018.00383","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Dendritic cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b350996f-152c-5b07-a28a-3243ae9e3a4d","evidence_kind":"source_excerpt","locator":"Lines 386-397","start_line":386,"end_line":397,"excerpt":"### pectin-dc-il6\nDM7 reduced stimulus-dependent IL-6 release from human dendritic cells; at lower tested concentrations DM75 did not reproduce this effect.\nCondition category: normal\nnutrient_topic: Pectin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Preparation structure affected how much the cells released an inflammatory messenger.\norganism: Homo sapiens\ntissue_or_cell_type: Dendritic cells\nexperimental_model: Receptor binding, reporter cells, human dendritic cells and mouse ileitis model\nlimitations: Model-specific. Binding and cytokine experiments do not establish prevention of human inflammatory disease. Lack of increased measured SCFAs does not exclude every microbial contribution.\nexposure: Lemon pectin DM7 versus DM75; cell exposures 1-100 micrograms/mL; mice 3 mg/day before doxorubicin\nevidence_span: {\"source_cache\": \"artifacts/pectin-research/29545800.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4b9e856ec2420c457b98f455ec243b742525b63d9382b69ef087456e0136cb42\", \"start_char\": 38875, \"end_char\": 39612, \"text_sha256\": \"8b75517328dfdbdd5a4290c5523e8136762b0456a8fd69af5e0e172edb168bda\"}\n[pectin-p29545800] Dietary Fiber Pectin Directly Blocks Toll-Like Receptor 2-1 and Prevents Doxorubicin-Induced Ileitis. 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