{"id":"a9355f77-f486-50ec-bc3e-6f2729defc28","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-erythrocyte-gsh","predicate":"induces","statement":"Vanadate exposure depleted intracellular nonprotein sulfhydryls while increasing extracellular glutathione in human erythrocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"acfcb8ad-a317-5813-a3af-99c674d3f130","mechanism_event_label":"Loss of an intracellular thiol pool can include export rather than only oxidation.","subject":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"object":{"id":"1ad15cf2-df32-5ea0-83f3-30317902aab7","slug":"human-erythrocyte-vanadate-gsh-efflux","display_name":"Glutathione efflux from vanadate-exposed human erythrocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"acfcb8ad-a317-5813-a3af-99c674d3f130","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-erythrocyte-gsh-event","event_type":"observed_relationship","label":"Loss of an intracellular thiol pool can include export rather than only oxidation.","description":"Vanadate exposure depleted intracellular nonprotein sulfhydryls while increasing extracellular glutathione in human erythrocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1ad15cf2-df32-5ea0-83f3-30317902aab7","slug":"human-erythrocyte-vanadate-gsh-efflux","display_name":"Glutathione efflux from vanadate-exposed human erythrocytes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated human erythrocytes; millimolar vanadate exposures up to four hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High ex-vivo concentrations; nonprotein sulfhydryl assays are not exclusively glutathione measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Loss of an intracellular thiol pool can include export rather than only oxidation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Efflux of glutathione and glutathione complexes from human erythrocytes in response to vanadate. · 2013 · https://pubmed.ncbi.nlm.nih.gov/22824382/ · DOI 10.1016/j.bcmd.2012.07.001","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c157c058-f42f-5c47-887b-8d3a2941d16f","evidence_kind":"source_excerpt","locator":"Lines 158-164","start_line":158,"end_line":164,"excerpt":"## vanadium-erythrocyte-gsh\nLoss of an intracellular thiol pool can include export rather than only oxidation.\nVanadate exposure depleted intracellular nonprotein sulfhydryls while increasing extracellular glutathione in human erythrocytes.\nModel: Isolated human erythrocytes; millimolar vanadate exposures up to four hours.\nLimitations: High ex-vivo concentrations; nonprotein sulfhydryl assays are not exclusively glutathione measurements.\nEvidence access: Primary abstract\nEfflux of glutathione and glutathione complexes from human erythrocytes in response to vanadate. · 2013 · https://pubmed.ncbi.nlm.nih.gov/22824382/ · DOI 10.1016/j.bcmd.2012.07.001","model_system":"Isolated human erythrocytes; millimolar vanadate exposures up to four hours.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"d36b30d972e756ca4d19b66f976980e206c65025bd4bbf4c127c17320bd019c2","revision_id":"70d42967-8032-5627-8670-f59841735e89","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}