{"id":"a90479b6-d5e6-5362-a29c-720be4fd879f","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-skin-barrier","predicate":"improves_tested","statement":"Histidine supplementation improved the measured barrier readout in human skin-equivalent experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b31fa1ca-9be3-56a1-8341-ff2e879143b6","mechanism_event_label":"The culture study also measured a functional barrier outcome.","subject":{"id":"44374451-3436-5136-a8ae-5dcc6d8c353b","slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"},"object":{"id":"2a3456cb-28b6-5364-91ed-15c4214b57f5","slug":"human-skin-equivalent-barrier","display_name":"Barrier function in human skin-equivalent culture","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b31fa1ca-9be3-56a1-8341-ff2e879143b6","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-skin-barrier-event","event_type":"observed_relationship","label":"The culture study also measured a functional barrier outcome.","description":"Histidine supplementation improved the measured barrier readout in human skin-equivalent experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"44374451-3436-5136-a8ae-5dcc6d8c353b","slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2a3456cb-28b6-5364-91ed-15c4214b57f5","slug":"human-skin-equivalent-barrier","display_name":"Barrier function in human skin-equivalent culture","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ec90da92-1bbd-56ff-86c1-c6a4bac32093","slug":"flg","display_name":"Human filaggrin / FLG","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human skin-equivalent in-vitro assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Separate from oral clinical outcomes; no direct proof of the entire mediation chain in patients.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Histidine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The culture study also measured a functional barrier outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Feeding filaggrin: effects of l-histidine supplementation in atopic dermatitis. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29042806/ · DOI 10.2147/CCID.S146760","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4e62a727-9cab-5d79-ae82-4f0018b49b7b","evidence_kind":"source_excerpt","locator":"Lines 418-424","start_line":418,"end_line":424,"excerpt":"## histidine-skin-barrier\nThe culture study also measured a functional barrier outcome.\nHistidine supplementation improved the measured barrier readout in human skin-equivalent experiments.\nModel: Human skin-equivalent in-vitro assay.\nLimitations: Separate from oral clinical outcomes; no direct proof of the entire mediation chain in patients.\nEvidence access: Primary full text\nFeeding filaggrin: effects of l-histidine supplementation in atopic dermatitis. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29042806/ · DOI 10.2147/CCID.S146760","model_system":"Human skin-equivalent in-vitro assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ce59e6c9-1213-523b-9d0b-400b7a81cd1c","stable_key":"import-911fb3c7-8cc3-5667-b677-5682fab67648","title":"L-Histidine: supply, catabolism, histamine, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"cb672530ec8b4a4542d0f3957e80ca76bb0449e9f988845b0f7681f623c4ec9b","revision_id":"19a7f26c-d9a1-5411-a81d-025f3d98a452","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}