{"id":"a90411ba-a174-5d12-b4c4-3039cf0497ce","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-mfsd5-uptake","predicate":"mediates_heterologous_uptake_of","statement":"Human HsMOT2/MFSD5 expression supported molybdate uptake in yeast, with an apparent affinity near 550 nM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8ab37e1c-de40-5dc4-9415-993c43ce4168","mechanism_event_label":"A human protein can carry molybdate when tested in yeast.","subject":{"id":"49b228c5-a2e8-56a3-82a9-de12dd6f3731","slug":"mfsd5","display_name":"Human MFSD5 / HsMOT2","entity_type_key":"protein"},"object":{"id":"18479412-e52f-5921-8b5a-ddacefcbe083","slug":"molybdate","display_name":"Molybdate / MoO4(2-)","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"8ab37e1c-de40-5dc4-9415-993c43ce4168","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-mfsd5-uptake-event","event_type":"biochemical_relationship","label":"A human protein can carry molybdate when tested in yeast.","description":"Human HsMOT2/MFSD5 expression supported molybdate uptake in yeast, with an apparent affinity near 550 nM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"36447b98-3392-559f-b76c-035726a2538e","slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"},"role":"element","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49b228c5-a2e8-56a3-82a9-de12dd6f3731","slug":"mfsd5","display_name":"Human MFSD5 / HsMOT2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"18479412-e52f-5921-8b5a-ddacefcbe083","slug":"molybdate","display_name":"Molybdate / MoO4(2-)","entity_type_key":"ion"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/21464289.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d2ba286dba58f70f8a9e9a4f61259329ed5f458d5c4b1e9cd13c64df916f300d\", \"start_char\": 0, \"end_char\": 1707, \"text_sha256\": \"d2ba286dba58f70f8a9e9a4f61259329ed5f458d5c4b1e9cd13c64df916f300d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HsMOT2 expressed in Saccharomyces cerevisiae, with separate algal experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Molybdate uptake assays and oxyanion comparisons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Establishes transport capacity in yeast; does not identify the dominant intestinal or renal transporter in people.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human protein in yeast; Chlamydomonas experiments separately","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A human protein can carry molybdate when tested in yeast.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p21464289] Algae and humans share a molybdate transporter. (2011). https://pubmed.ncbi.nlm.nih.gov/21464289/ DOI: 10.1073/pnas.1100700108","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Heterologous membrane transport","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0fc5c28a-f1af-598a-82f5-6fde6e1f9758","evidence_kind":"source_excerpt","locator":"Lines 196-207","start_line":196,"end_line":207,"excerpt":"### mo-mfsd5-uptake\nHuman HsMOT2/MFSD5 expression supported molybdate uptake in yeast, with an apparent affinity near 550 nM.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A human protein can carry molybdate when tested in yeast.\norganism: Human protein in yeast; Chlamydomonas experiments separately\ntissue_or_cell_type: Heterologous membrane transport\nexperimental_model: Human HsMOT2 expressed in Saccharomyces cerevisiae, with separate algal experiments\nlimitations: Establishes transport capacity in yeast; does not identify the dominant intestinal or renal transporter in people.\nexposure: Molybdate uptake assays and oxyanion comparisons\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/21464289.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d2ba286dba58f70f8a9e9a4f61259329ed5f458d5c4b1e9cd13c64df916f300d\", \"start_char\": 0, \"end_char\": 1707, \"text_sha256\": \"d2ba286dba58f70f8a9e9a4f61259329ed5f458d5c4b1e9cd13c64df916f300d\"}\n[mo-p21464289] Algae and humans share a molybdate transporter. (2011). https://pubmed.ncbi.nlm.nih.gov/21464289/ DOI: 10.1073/pnas.1100700108","model_system":"Human HsMOT2 expressed in Saccharomyces cerevisiae, with separate algal experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p21464289] Algae and humans share a molybdate transporter. (2011). https://pubmed.ncbi.nlm.nih.gov/21464289/ DOI: 10.1073/pnas.1100700108","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1aa6da60-8284-5252-8dd9-ac2250d5ced5","stable_key":"import-dc8975b1-95ff-5d9b-be17-a1c04610cca7","title":"Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"143147eb9ac7fe750bdd33bb50d7773bc0cd49cc94faf219aebce3c3d760dd8f","revision_id":"90c2239d-290b-5e16-815a-f94d30569ebd","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}