{"id":"a9013fd4-0c0c-53f8-8ee0-214e420d9333","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-high-dose-phe","predicate":"dose_dependently_increases","statement":"In 18 fasted adults across 100, 150 and 200 mg/kg cohorts, mean plasma phenylalanine peaks were 20.3, 35.1 and 48.7 micromol/dL.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ddc2268c-1c2d-5003-8a24-64c97f762682","mechanism_event_label":"Large loads produce larger amino-acid excursions.","subject":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"object":{"id":"53c53d5a-5af6-5901-ba09-bccf1f416178","slug":"human-plasma-phenylalanine","display_name":"Human plasma phenylalanine concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ddc2268c-1c2d-5003-8a24-64c97f762682","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-high-dose-phe-event","event_type":"observed_relationship","label":"Large loads produce larger amino-acid excursions.","description":"In 18 fasted adults across 100, 150 and 200 mg/kg cohorts, mean plasma phenylalanine peaks were 20.3, 35.1 and 48.7 micromol/dL.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"53c53d5a-5af6-5901-ba09-bccf1f416178","slug":"human-plasma-phenylalanine","display_name":"Human plasma phenylalanine concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Six people per dose; aspartame in orange juice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Healthy subjects, not PKU; no direct neurotransmitter assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Large loads produce larger amino-acid excursions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Plasma and erythrocyte concentrations of free amino acids in adult humans administered abuse doses of aspartame. · 1981 · https://pubmed.ncbi.nlm.nih.gov/7230277/ · DOI 10.1080/15287398109529980","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"c7353f67-53c2-56c6-af16-17f872930442","evidence_kind":"source_excerpt","locator":"Lines 138-144","start_line":138,"end_line":144,"excerpt":"## aspartame-high-dose-phe\nLarge loads produce larger amino-acid excursions.\nIn 18 fasted adults across 100, 150 and 200 mg/kg cohorts, mean plasma phenylalanine peaks were 20.3, 35.1 and 48.7 micromol/dL.\nModel: Six people per dose; aspartame in orange juice.\nLimitations: Healthy subjects, not PKU; no direct neurotransmitter assay.\nEvidence access: Primary abstract\nPlasma and erythrocyte concentrations of free amino acids in adult humans administered abuse doses of aspartame. · 1981 · https://pubmed.ncbi.nlm.nih.gov/7230277/ · DOI 10.1080/15287398109529980","model_system":"Six people per dose; aspartame in orange juice.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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