{"id":"a8c0cf29-2cbd-51b2-a812-f0476865c0c6","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:permeation-allicin-human-rbc-entry","predicate":"reported_relationship","statement":"Allicin permeated the tested membrane and reached thiols on the other side.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"e88e349b-b921-5af0-9424-cecb63c11337","mechanism_event_label":"Allicin permeated the tested membrane and reached thiols on the other side.","subject":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"object":{"id":"78e92e06-1a74-5e93-a2c0-e0626dd29d88","slug":"allicin-human-rbc-entry","display_name":"Allicin entry into isolated human erythrocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e88e349b-b921-5af0-9424-cecb63c11337","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:permeation-allicin-human-rbc-entry-event","event_type":"biochemical_relationship","label":"Allicin permeated the tested membrane and reached thiols on the other side.","description":"**Membrane entry.** Allicin crossed phospholipid vesicles and human erythrocyte membranes and reacted with enclosed thiols. The investigators did not find membrane leakage, fusion, or aggregation in those assays. Permeation supports intracellular target access during direct exposure; it does not establish oral delivery to a distant tissue or make transport and resistance irrelevant in every organism. [Miron 2000](https://pubmed.ncbi.nlm.nih.gov/10631291/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"78e92e06-1a74-5e93-a2c0-e0626dd29d88","slug":"allicin-human-rbc-entry","display_name":"Allicin entry into isolated human erythrocytes","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, exact concentrations or species-specific attribution remain unextracted unless explicitly stated in the abstract or separately verified publisher text.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated human red blood cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Allicin permeated the tested membrane and reached thiols on the other side.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The mode of action of allicin: its ready permeability through phospholipid membranes may contribute to its biological activity. | 2000 | DOI 10.1016/s0005-2736(99)00174-1 | PMID 10631291 | https://pubmed.ncbi.nlm.nih.gov/10631291/ | https://doi.org/10.1016/s0005-2736(99)00174-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 27-27; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"23b5a7a8-f4ed-5ba0-b210-d3b8aaa84ecd","evidence_kind":"source_excerpt","locator":"Lines 27-27","start_line":27,"end_line":27,"excerpt":"**Membrane entry.** Allicin crossed phospholipid vesicles and human erythrocyte membranes and reacted with enclosed thiols. The investigators did not find membrane leakage, fusion, or aggregation in those assays. Permeation supports intracellular target access during direct exposure; it does not establish oral delivery to a distant tissue or make transport and resistance irrelevant in every organism. [Miron 2000](https://pubmed.ncbi.nlm.nih.gov/10631291/)","model_system":"Isolated human red blood cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}