{"id":"a8836fec-e839-50b6-9930-d8b633ebd642","stable_key":"b26c9d01-98fd-50d3-8f39-44db46961ee5:acetate-maternal-alcohol-fetal-brain","predicate":"increases","statement":"In a pregnant mouse, exposure to labelled alcohol resulted in the incorporation of labelled acetyl groups into gestating fetal brains.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"eea8cbd2-2ba1-531d-ac0d-a91523df052f","mechanism_event_label":"The same atoms cross into the fetus and land on the chromatin of its developing brain.","subject":{"id":"e653a47d-0723-5479-9572-6ed51aa8d5b0","slug":"ethanol","display_name":"Ethanol","entity_type_key":"drug"},"object":{"id":"7642977a-8643-5932-98b6-70a30f4765d6","slug":"fetal-brain-histone-acetylation","display_name":"Acetyl groups incorporated into gestating fetal brains","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"eea8cbd2-2ba1-531d-ac0d-a91523df052f","stable_key":"b26c9d01-98fd-50d3-8f39-44db46961ee5:acetate-maternal-alcohol-fetal-brain-event","event_type":"biochemical_relationship","label":"The same atoms cross into the fetus and land on the chromatin of its developing brain.","description":"In a pregnant mouse, exposure to labelled alcohol resulted in the incorporation of labelled acetyl groups into gestating fetal brains.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eb21419d-28fe-5a9f-b934-3eb430ad89e0","slug":"alcohol-derived-acetate","display_name":"Acetate derived from hepatic alcohol metabolism","entity_type_key":"cellular_process"},"role":"carrier","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"64044850-0d45-5593-9a51-fa645679f99b","slug":"acss2","display_name":"Human acetyl-CoA synthetase 2 / ACSS2","entity_type_key":"protein"},"role":"required_enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e653a47d-0723-5479-9572-6ed51aa8d5b0","slug":"ethanol","display_name":"Ethanol","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7642977a-8643-5932-98b6-70a30f4765d6","slug":"fetal-brain-histone-acetylation","display_name":"Acetyl groups incorporated into gestating fetal brains","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/acetate-research/31645761.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a7a812a410fd8be3bad8512ab46d4b8a7fd3329d1552ee30965b66860eeb2b2f\", \"start_char\": 0, \"end_char\": 1646, \"text_sha256\": \"a7a812a410fd8be3bad8512ab46d4b8a7fd3329d1552ee30965b66860eeb2b2f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"In vivo stable-isotope labelling in mice, with primary hippocampal neurons and behavioural testing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Labelled alcohol or heavy-labelled acetate administered in vivo, with ACSS2 inhibition and deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Isotope labelling traces the actual carbon atoms onto histones, which is stronger than correlating acetylation with exposure. A mouse study; the fetal result is a single reported exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Acetic acid research collection; topical membership is not evidence of a direct clinical effect, and the ingested acid is recorded separately from the circulating acetate anion.","comparator":null,"unit":null,"notes":"","entity":{"slug":"acetic-acid","display_name":"Acetic acid","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The same atoms cross into the fetus and land on the chromatin of its developing brain.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[acetate-p31645761] Alcohol metabolism contributes to brain histone acetylation. (2019). https://pubmed.ncbi.nlm.nih.gov/31645761/ DOI: 10.1038/s41586-019-1700-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brain and gestating fetus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"9def8000-0671-51e8-9e63-9c034878b5a3","evidence_kind":"source_excerpt","locator":"Lines 628-639","start_line":628,"end_line":639,"excerpt":"### acetate-maternal-alcohol-fetal-brain\nIn a pregnant mouse, exposure to labelled alcohol resulted in the incorporation of labelled acetyl groups into gestating fetal brains.\nCondition category: biomarker_context\nnutrient_topic: Acetic acid research collection; topical membership is not evidence of a direct clinical effect, and the ingested acid is recorded separately from the circulating acetate anion.\nplain_language: The same atoms cross into the fetus and land on the chromatin of its developing brain.\norganism: Mouse\ntissue_or_cell_type: Brain and gestating fetus\nexperimental_model: In vivo stable-isotope labelling in mice, with primary hippocampal neurons and behavioural testing\nlimitations: Isotope labelling traces the actual carbon atoms onto histones, which is stronger than correlating acetylation with exposure. A mouse study; the fetal result is a single reported exposure.\nexposure: Labelled alcohol or heavy-labelled acetate administered in vivo, with ACSS2 inhibition and deletion\nevidence_span: {\"source_cache\": \"artifacts/acetate-research/31645761.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a7a812a410fd8be3bad8512ab46d4b8a7fd3329d1552ee30965b66860eeb2b2f\", \"start_char\": 0, \"end_char\": 1646, \"text_sha256\": \"a7a812a410fd8be3bad8512ab46d4b8a7fd3329d1552ee30965b66860eeb2b2f\"}\n[acetate-p31645761] Alcohol metabolism contributes to brain histone acetylation. (2019). https://pubmed.ncbi.nlm.nih.gov/31645761/ DOI: 10.1038/s41586-019-1700-7","model_system":"In vivo stable-isotope labelling in mice, with primary hippocampal neurons and behavioural testing","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [acetate-p31645761] Alcohol metabolism contributes to brain histone acetylation. (2019). https://pubmed.ncbi.nlm.nih.gov/31645761/ DOI: 10.1038/s41586-019-1700-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"8356baaf-173c-5549-80cd-bbbb2007ffb5","stable_key":"import-b26c9d01-98fd-50d3-8f39-44db46961ee5","title":"Acetic acid: the ingested acid, the receptors acetate binds, the acetyl-CoA it becomes, and the acetyl groups that reach histones (2026-09-21)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"1441d249b16db25c0d488407e5cc881222acffc2f16af88cb3197d9d5697bccc","revision_id":"9bfeb605-f589-5e99-84b9-e56968a3bc1f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}