{"id":"a8537ed5-ef05-55c1-bea0-fe451cebc4b2","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-quercetin-oat3","predicate":"inhibits","statement":"Quercetin inhibited OAT3-mediated steviol-glucuronide uptake, with IC50 1.8 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"3c61462f-c250-5621-aaa9-65d4e1fb5018","mechanism_event_label":"A food-derived flavonoid intersects the metabolite clearance route.","subject":{"id":"2e17b6b4-ba3c-5167-89ed-a667f3cb808e","slug":"quercetin","display_name":"Quercetin","entity_type_key":"small_molecule"},"object":{"id":"5e4ab3f9-5b15-59f2-94e6-24ca9bda87bb","slug":"human-oat3-steviol-glucuronide-uptake","display_name":"Human OAT3-mediated steviol-glucuronide uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3c61462f-c250-5621-aaa9-65d4e1fb5018","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-quercetin-oat3-event","event_type":"observed_relationship","label":"A food-derived flavonoid intersects the metabolite clearance route.","description":"Quercetin inhibited OAT3-mediated steviol-glucuronide uptake, with IC50 1.8 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2e17b6b4-ba3c-5167-89ed-a667f3cb808e","slug":"quercetin","display_name":"Quercetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5e4ab3f9-5b15-59f2-94e6-24ca9bda87bb","slug":"human-oat3-steviol-glucuronide-uptake","display_name":"Human OAT3-mediated steviol-glucuronide uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4606352d-ad2e-580f-87eb-e0ccf0d02fd9","slug":"slc22a8","display_name":"Human organic anion transporter 3 / SLC22A8","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f008b74d-164b-5663-a72e-04a48fe7a8fb","slug":"steviol-glucuronide","display_name":"Steviol glucuronide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"In vitro transport inhibition assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Free tissue exposure and a human pharmacokinetic interaction were not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A food-derived flavonoid intersects the metabolite clearance route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transmembrane transport of steviol glucuronide and its potential interaction with selected drugs and natural compounds. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26525112/ · DOI 10.1016/j.fct.2015.10.011","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f0b1e344-a676-51b6-ad7c-92615e7ad76b","evidence_kind":"source_excerpt","locator":"Lines 130-136","start_line":130,"end_line":136,"excerpt":"## stevia-quercetin-oat3\nA food-derived flavonoid intersects the metabolite clearance route.\nQuercetin inhibited OAT3-mediated steviol-glucuronide uptake, with IC50 1.8 micromolar.\nModel: In vitro transport inhibition assay.\nLimitations: Free tissue exposure and a human pharmacokinetic interaction were not established.\nEvidence access: Primary abstract\nTransmembrane transport of steviol glucuronide and its potential interaction with selected drugs and natural compounds. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26525112/ · DOI 10.1016/j.fct.2015.10.011","model_system":"In vitro transport inhibition assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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