{"id":"a809bc89-8a6d-58f8-9221-8cd7f9866ea7","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-gad2-gaba","predicate":"catalyzes","statement":"PLP-bound human GAD65 catalyzes glutamate decarboxylation to GABA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5becf53a-8665-5c72-a1e0-568f8ac42342","mechanism_event_label":"Activated B6 helps this enzyme make GABA.","subject":{"id":"453252f4-83ef-5064-a3f7-21f976f9a3f3","slug":"gad2","display_name":"Human glutamate decarboxylase 65 / GAD2","entity_type_key":"protein"},"object":{"id":"d5b98fa8-0612-5a5a-aa3c-6cfd8ff88867","slug":"gaba","display_name":"Gamma-aminobutyric acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"5becf53a-8665-5c72-a1e0-568f8ac42342","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-gad2-gaba-event","event_type":"biochemical_relationship","label":"Activated B6 helps this enzyme make GABA.","description":"PLP-bound human GAD65 catalyzes glutamate decarboxylation to GABA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"453252f4-83ef-5064-a3f7-21f976f9a3f3","slug":"gad2","display_name":"Human glutamate decarboxylase 65 / GAD2","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d5b98fa8-0612-5a5a-aa3c-6cfd8ff88867","slug":"gaba","display_name":"Gamma-aminobutyric acid","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Recombinant human GAD isoforms; crystallography and enzyme kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Glutamate turnover in recombinant enzyme assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Recombinant chemistry does not establish a human supplementation response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Activated B6 helps this enzyme make GABA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fenalti-2007-gad] GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop (2007). https://doi.org/10.1038/nsmb1228 DOI: 10.1038/nsmb1228","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"75ccb940-8786-5bd8-bf86-953d54143e84","evidence_kind":"source_excerpt","locator":"Lines 961-971","start_line":961,"end_line":971,"excerpt":"### b6-neuro-gad2-gaba\nPLP-bound human GAD65 catalyzes glutamate decarboxylation to GABA.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Activated B6 helps this enzyme make GABA.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Recombinant human GAD isoforms; crystallography and enzyme kinetics\nlimitations: Recombinant chemistry does not establish a human supplementation response.\nexposure: Glutamate turnover in recombinant enzyme assays.\n[fenalti-2007-gad] GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop (2007). https://doi.org/10.1038/nsmb1228 DOI: 10.1038/nsmb1228","model_system":"Recombinant human GAD isoforms; crystallography and enzyme kinetics","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fenalti-2007-gad] GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop (2007). https://doi.org/10.1038/nsmb1228 DOI: 10.1038/nsmb1228","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}