{"id":"a8035d9a-2712-5af1-84a2-22a12d9c4d24","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-bcat1-leucine","predicate":"catalyzes-transamination-of","statement":"Human BCAT1 transfers the leucine amino group to 2-oxoglutarate, producing alpha-ketoisocaproate and glutamate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8cba0627-b3aa-5109-bcb6-2ba3da824798","mechanism_event_label":"B6-dependent transamination starts leucine processing and transfers nitrogen to glutamate.","subject":{"id":"7723e640-d4b2-5cc2-a8ed-ee6f3a1050e5","slug":"bcat1","display_name":"Human cytosolic branched-chain aminotransferase / BCAT1","entity_type_key":"protein"},"object":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"8cba0627-b3aa-5109-bcb6-2ba3da824798","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-bcat1-leucine-event","event_type":"biochemical_relationship","label":"B6-dependent transamination starts leucine processing and transfers nitrogen to glutamate.","description":"Human BCAT1 transfers the leucine amino group to 2-oxoglutarate, producing alpha-ketoisocaproate and glutamate.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"7723e640-d4b2-5cc2-a8ed-ee6f3a1050e5","slug":"bcat1","display_name":"Human cytosolic branched-chain aminotransferase / BCAT1","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c8dec85c-c85d-5a55-ba85-ad3c1b3e332b","slug":"pyridoxamine-phosphate","display_name":"Pyridoxamine 5-prime-phosphate","entity_type_key":"small_molecule"},"role":"cycling cofactor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"role":"amino donor","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"amino acceptor","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"bbdfd11c-42dd-520e-8a1f-7fe3d19e3e0e","slug":"alpha-ketoisocaproate","display_name":"4-Methyl-2-oxopentanoate / alpha-ketoisocaproate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Essential amino-acid nitrogen transfer; net direction depends on substrate/product conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human BCAT1 and BCAT2; pre-steady-state kinetics and structures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified-enzyme evidence does not define dietary requirements or cellular PLP thresholds.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"B6-dependent transamination starts leucine processing and transfers nitrogen to glutamate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-bcat1-2005] Structural determinants for branched-chain aminotransferase isozyme-specific inhibition by the anticonvulsant drug gabapentin (2005). https://pubmed.ncbi.nlm.nih.gov/16141215/ DOI: 10.1074/jbc.M506486200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4387e679-da17-5349-9b2e-30844c58aae5","evidence_kind":"source_excerpt","locator":"Lines 717-727","start_line":717,"end_line":727,"excerpt":"### b6-met-bcat1-leucine\nHuman BCAT1 transfers the leucine amino group to 2-oxoglutarate, producing alpha-ketoisocaproate and glutamate.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: B6-dependent transamination starts leucine processing and transfers nitrogen to glutamate.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Recombinant human BCAT1 and BCAT2; pre-steady-state kinetics and structures\nlimitations: Purified-enzyme evidence does not define dietary requirements or cellular PLP thresholds.\ncross_nutrient: Essential amino-acid nitrogen transfer; net direction depends on substrate/product conditions.\n[b6-bcat1-2005] Structural determinants for branched-chain aminotransferase isozyme-specific inhibition by the anticonvulsant drug gabapentin (2005). https://pubmed.ncbi.nlm.nih.gov/16141215/ DOI: 10.1074/jbc.M506486200","model_system":"Recombinant human BCAT1 and BCAT2; pre-steady-state kinetics and structures","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-bcat1-2005] Structural determinants for branched-chain aminotransferase isozyme-specific inhibition by the anticonvulsant drug gabapentin (2005). https://pubmed.ncbi.nlm.nih.gov/16141215/ DOI: 10.1074/jbc.M506486200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}