{"id":"a799c453-f04a-5e4d-bb84-71844a325ba5","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-gls2-energy","predicate":"supports_tested","statement":"GLS2 manipulation linked increased glutamate and 2-oxoglutarate production with increased respiration and ATP generation in the tested human cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"eb050904-cb14-5937-91e4-f3a25c3667b4","mechanism_event_label":"The generated carbon skeleton can support mitochondrial metabolism.","subject":{"id":"6981fb90-5bc3-5c2a-9f22-f1974e287f38","slug":"gls2","display_name":"Human glutaminase 2 / GLS2","entity_type_key":"protein"},"object":{"id":"44ee5896-f595-5f24-a4dc-ac07db090a2d","slug":"human-gls2-cell-respiration","display_name":"Respiration and ATP production in human GLS2 experiments","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"eb050904-cb14-5937-91e4-f3a25c3667b4","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-gls2-energy-event","event_type":"observed_relationship","label":"The generated carbon skeleton can support mitochondrial metabolism.","description":"GLS2 manipulation linked increased glutamate and 2-oxoglutarate production with increased respiration and ATP generation in the tested human cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6981fb90-5bc3-5c2a-9f22-f1974e287f38","slug":"gls2","display_name":"Human glutaminase 2 / GLS2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"44ee5896-f595-5f24-a4dc-ac07db090a2d","slug":"human-gls2-cell-respiration","display_name":"Respiration and ATP production in human GLS2 experiments","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human-cell GLS2 expression/perturbation study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The effect depends on cell context; it is not a clinical energy claim.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The generated carbon skeleton can support mitochondrial metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20378837/ · DOI 10.1073/pnas.1001006107","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"407d2a7b-0e0b-58fb-88e7-fb3389e493b1","evidence_kind":"source_excerpt","locator":"Lines 218-224","start_line":218,"end_line":224,"excerpt":"## glutamate-gls2-energy\nThe generated carbon skeleton can support mitochondrial metabolism.\nGLS2 manipulation linked increased glutamate and 2-oxoglutarate production with increased respiration and ATP generation in the tested human cells.\nModel: Human-cell GLS2 expression/perturbation study.\nLimitations: The effect depends on cell context; it is not a clinical energy claim.\nEvidence access: Primary abstract\nGlutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20378837/ · DOI 10.1073/pnas.1001006107","model_system":"Human-cell GLS2 expression/perturbation study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3da83bd75fddeb96d17af72d7e2ae04344779957650f266521f6c8ebd59fb54f","revision_id":"55196c72-94e4-540a-a869-81d35647b04a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}