{"id":"a7961e18-29d1-5590-96f5-6a8880b53cac","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-glo2-recycles","predicate":"hydrolyzes","statement":"Human glyoxalase II hydrolyzes S-D-lactoylglutathione to GSH and D-lactate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cb966816-01ac-5fab-aae8-3d70dd738460","mechanism_event_label":"This clearance route regenerates glutathione instead of permanently consuming it.","subject":{"id":"3deb69c4-1b89-5f34-9bad-dcb293418173","slug":"hagh","display_name":"Human glyoxalase II / HAGH","entity_type_key":"protein"},"object":{"id":"60da82b9-4ed2-52e8-ad0d-8152663ff5af","slug":"s-d-lactoylglutathione","display_name":"S-D-Lactoylglutathione","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"cb966816-01ac-5fab-aae8-3d70dd738460","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-glo2-recycles-event","event_type":"biochemical_relationship","label":"This clearance route regenerates glutathione instead of permanently consuming it.","description":"Human glyoxalase II hydrolyzes S-D-lactoylglutathione to GSH and D-lactate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"regenerated_product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"400687c3-8d8b-5488-96ab-e594d3c2182c","slug":"d-lactate","display_name":"D-Lactate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3deb69c4-1b89-5f34-9bad-dcb293418173","slug":"hagh","display_name":"Human glyoxalase II / HAGH","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"60da82b9-4ed2-52e8-ad0d-8152663ff5af","slug":"s-d-lactoylglutathione","display_name":"S-D-Lactoylglutathione","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/glutathione-research/10508780.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2a3bc96920fd7c09bc46bfbdd6ade9698bb3dd421e0867023dec7679628d04eb\", \"start_char\": 0, \"end_char\": 1432, \"text_sha256\": \"2a3bc96920fd7c09bc46bfbdd6ade9698bb3dd421e0867023dec7679628d04eb\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Crystal structures of free and ligand-bound GLO2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Thioester hydrolysis and substrate-analogue structure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The structure contained a binuclear zinc site; metal occupancy in this preparation is not a universal in-vivo metal assignment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glutathione research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This clearance route regenerates glutathione instead of permanently consuming it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[glutathione-p10508780] Crystal structure of human glyoxalase II and its complex with a glutathione thiolester substrate analogue. (1999). https://pubmed.ncbi.nlm.nih.gov/10508780/ DOI: 10.1016/s0969-2126(99)80174-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified glyoxalase II","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ddff7070-9c04-5c0c-802d-661cb9424ebd","evidence_kind":"source_excerpt","locator":"Lines 905-916","start_line":905,"end_line":916,"excerpt":"### glutathione-glo2-recycles\nHuman glyoxalase II hydrolyzes S-D-lactoylglutathione to GSH and D-lactate.\nCondition category: normal\nnutrient_topic: Glutathione research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This clearance route regenerates glutathione instead of permanently consuming it.\norganism: Human\ntissue_or_cell_type: Purified glyoxalase II\nexperimental_model: Crystal structures of free and ligand-bound GLO2\nlimitations: The structure contained a binuclear zinc site; metal occupancy in this preparation is not a universal in-vivo metal assignment.\nexposure: Thioester hydrolysis and substrate-analogue structure\nevidence_span: {\"source_cache\": \"artifacts/glutathione-research/10508780.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2a3bc96920fd7c09bc46bfbdd6ade9698bb3dd421e0867023dec7679628d04eb\", \"start_char\": 0, \"end_char\": 1432, \"text_sha256\": \"2a3bc96920fd7c09bc46bfbdd6ade9698bb3dd421e0867023dec7679628d04eb\"}\n[glutathione-p10508780] Crystal structure of human glyoxalase II and its complex with a glutathione thiolester substrate analogue. 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