{"id":"a77d2c17-50cd-58bc-9d21-1e98153deb03","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-tubulin-retyrosination","predicate":"adds","statement":"Recombinant chicken TTL incorporated radiolabeled tyrosine into mammalian brain alpha-tubulin in ATP/Mg-containing assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"83e5a141-5399-53b1-8c1a-ebcbc94a7153","mechanism_event_label":"Tyrosine can be attached to an existing protein after translation.","subject":{"id":"fc12a174-0b8a-5ff0-a6ca-d62a9af11118","slug":"chicken-ttl","display_name":"Chicken tubulin tyrosine ligase / Ttl","entity_type_key":"protein"},"object":{"id":"2538baea-6c5b-5fc9-b4ab-385085073ba3","slug":"mammalian-brain-tubulin-terminal-tyrosine","display_name":"Bovine/porcine brain alpha-tubulin terminal tyrosine","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"83e5a141-5399-53b1-8c1a-ebcbc94a7153","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-tubulin-retyrosination-event","event_type":"observed_relationship","label":"Tyrosine can be attached to an existing protein after translation.","description":"Recombinant chicken TTL incorporated radiolabeled tyrosine into mammalian brain alpha-tubulin in ATP/Mg-containing assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fc12a174-0b8a-5ff0-a6ca-d62a9af11118","slug":"chicken-ttl","display_name":"Chicken tubulin tyrosine ligase / Ttl","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2538baea-6c5b-5fc9-b4ab-385085073ba3","slug":"mammalian-brain-tubulin-terminal-tyrosine","display_name":"Bovine/porcine brain alpha-tubulin terminal tyrosine","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"0516476b-cb34-5a25-ac87-db3657d29c92","slug":"protein-bound-tyrosine","display_name":"Protein-bound L-tyrosine residue","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text; Methods and structure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chicken enzyme with bovine/porcine tubulin; 400 micromolar ATP, 2.4 millimolar MgCl2 and 5 micromolar labeled tyrosine in activity assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mixed-species reconstitution is not a human supplementation experiment. This is retyrosination, not tyrosine phosphorylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Tyrosine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Tyrosine can be attached to an existing protein after translation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structural basis of tubulin tyrosination by tubulin tyrosine ligase. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23358242/ · DOI 10.1083/jcb.201211017","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b86e8107-3a22-5f91-b873-4da152081b83","evidence_kind":"source_excerpt","locator":"Lines 316-322","start_line":316,"end_line":322,"excerpt":"## l-tyrosine-tubulin-retyrosination\nTyrosine can be attached to an existing protein after translation.\nRecombinant chicken TTL incorporated radiolabeled tyrosine into mammalian brain alpha-tubulin in ATP/Mg-containing assays.\nModel: Chicken enzyme with bovine/porcine tubulin; 400 micromolar ATP, 2.4 millimolar MgCl2 and 5 micromolar labeled tyrosine in activity assays.\nLimitations: Mixed-species reconstitution is not a human supplementation experiment. This is retyrosination, not tyrosine phosphorylation.\nEvidence access: Primary full text; Methods and structure\nStructural basis of tubulin tyrosination by tubulin tyrosine ligase. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23358242/ · DOI 10.1083/jcb.201211017","model_system":"Chicken enzyme with bovine/porcine tubulin; 400 micromolar ATP, 2.4 millimolar MgCl2 and 5 micromolar labeled tyrosine in activity assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"12917df2-c6e0-5b61-850f-dbff6d4b4d30","stable_key":"import-63ce713e-6aea-59f6-9896-ca30e010b2ce","title":"L-Tyrosine: catecholamines, thyroid chemistry, pigment, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7777f4440a8bfb14a04ff73e73392ccc45d8ee89f7853ecbcc0027d6f563b8a0","revision_id":"e1206111-3a75-5809-b8e9-231ff809ff1e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}