{"id":"a6b1dbd5-1505-5837-bf73-35b3d115f097","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-estrogen-growth-inhibition","predicate":"inhibits_in_estrogen_stimulated_cells","statement":"DIM inhibited estradiol-induced MCF-7 proliferation in the tested estrogen-stimulated system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"237149e2-52d0-5bef-bf34-580207536212","mechanism_event_label":"Its effect depended on the hormonal conditions being tested.","subject":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"object":{"id":"d3daee2b-acdd-5fc7-b96d-4439bda86b74","slug":"cell-proliferation","display_name":"Cell proliferation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"237149e2-52d0-5bef-bf34-580207536212","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-estrogen-growth-inhibition-event","event_type":"biochemical_relationship","label":"Its effect depended on the hormonal conditions being tested.","description":"DIM inhibited estradiol-induced MCF-7 proliferation in the tested estrogen-stimulated system.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0ca53c4b-a806-51f4-b027-9420d7c59d5d","slug":"17-beta-estradiol","display_name":"17-beta-Estradiol","entity_type_key":"small_molecule"},"role":"stimulus","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b03c0784-d18d-58e3-99c5-2bcfed07028b","slug":"esr1","display_name":"Human estrogen receptor alpha / ESR1","entity_type_key":"protein"},"role":"estrogen_signaling_context","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d3daee2b-acdd-5fc7-b96d-4439bda86b74","slug":"cell-proliferation","display_name":"Cell proliferation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dim-research/9771935.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2f0967e62f0757c6ff4e68f7f7f673b6ba12ec581cbc7bd5c5594bdbf1964799\", \"start_char\": 0, \"end_char\": 1556, \"text_sha256\": \"2f0967e62f0757c6ff4e68f7f7f673b6ba12ec581cbc7bd5c5594bdbf1964799\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Ligand binding, nuclear complex, transcription and growth assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"DIM 10-50 micromolar in cells; rodent dosing separately","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preclinical evidence. Estrogen exposure, tissue and concentrations affect the response; no human cancer-treatment efficacy established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human MCF-7 cells and rat mammary-tumor model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Its effect depended on the hormonal conditions being tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dim-p9771935] Aryl hydrocarbon receptor-mediated antiestrogenic and antitumorigenic activity of diindolylmethane. 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Estrogen exposure, tissue and concentrations affect the response; no human cancer-treatment efficacy established.\nexposure: DIM 10-50 micromolar in cells; rodent dosing separately\nevidence_span: {\"source_cache\": \"artifacts/dim-research/9771935.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2f0967e62f0757c6ff4e68f7f7f673b6ba12ec581cbc7bd5c5594bdbf1964799\", \"start_char\": 0, \"end_char\": 1556, \"text_sha256\": \"2f0967e62f0757c6ff4e68f7f7f673b6ba12ec581cbc7bd5c5594bdbf1964799\"}\n[dim-p9771935] Aryl hydrocarbon receptor-mediated antiestrogenic and antitumorigenic activity of diindolylmethane. 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