{"id":"a69d517a-3469-511b-83ae-aa58a26c51a5","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-bacterial-iron-step","predicate":"sulfurizes","statement":"Reconstituted Mycobacterium smegmatis biosynthesis required an iron(II)-dependent oxidative sulfurization step.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5cb26da6-bfe2-525b-a140-64a7ad3310e8","mechanism_event_label":"Iron supports a microbial synthesis enzyme.","subject":{"id":"c1b6e6e0-7a66-5755-b162-18fef829ca21","slug":"mycobacterium-smegmatis-egtb","display_name":"Mycobacterium smegmatis EgtB","entity_type_key":"protein"},"object":{"id":"b3583649-cbbd-50f1-be33-26b1ee0dd6ab","slug":"hercynine","display_name":"Hercynine / N-alpha-trimethylhistidine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"5cb26da6-bfe2-525b-a140-64a7ad3310e8","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-bacterial-iron-step-event","event_type":"observed_relationship","label":"Iron supports a microbial synthesis enzyme.","description":"Reconstituted Mycobacterium smegmatis biosynthesis required an iron(II)-dependent oxidative sulfurization step.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c1b6e6e0-7a66-5755-b162-18fef829ca21","slug":"mycobacterium-smegmatis-egtb","display_name":"Mycobacterium smegmatis EgtB","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b3583649-cbbd-50f1-be33-26b1ee0dd6ab","slug":"hercynine","display_name":"Hercynine / N-alpha-trimethylhistidine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"59d6d1cd-df32-5b58-b950-3188bc7b95d6","slug":"iron-ii","display_name":"Ferrous iron","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"8a0617df-cacc-55b1-b5ff-3b28afa12f20","slug":"hercynyl-gamma-glutamylcysteine-sulfoxide","display_name":"Hercynyl-gamma-glutamylcysteine sulfoxide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified bacterial pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not mean iron supplements increase human synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Iron supports a microbial synthesis enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"In vitro reconstitution of Mycobacterial ergothioneine biosynthesis. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20420449/ · DOI 10.1021/ja101721e","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dbf09b9d-f72f-51cc-b966-3ac1da2a2626","evidence_kind":"source_excerpt","locator":"Lines 176-182","start_line":176,"end_line":182,"excerpt":"## ergothioneine-bacterial-iron-step\nIron supports a microbial synthesis enzyme.\nReconstituted Mycobacterium smegmatis biosynthesis required an iron(II)-dependent oxidative sulfurization step.\nModel: Purified bacterial pathway.\nLimitations: Does not mean iron supplements increase human synthesis.\nEvidence access: Primary abstract\nIn vitro reconstitution of Mycobacterial ergothioneine biosynthesis. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20420449/ · DOI 10.1021/ja101721e","model_system":"Purified bacterial pathway.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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