{"id":"a63447fe-330f-5cd1-b3b8-13d4c85ee991","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-rat-ptp1b-response","predicate":"reduces_activity_and_abundance_of","statement":"Obese rats receiving chromium picolinate had lower muscle PTP1B abundance and lower activity after correction for protein content.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8bf791ac-35f7-56c9-b395-f1783efcbf10","mechanism_event_label":"A protein that can oppose insulin signaling was reduced in this rat model.","subject":{"id":"89d5d39c-2663-5e0c-8eae-562a5b25a47d","slug":"chromium-iii-picolinate","display_name":"Chromium(III) picolinate / CrPic","entity_type_key":"chemical_species"},"object":{"id":"286c37b4-6064-5a17-b556-81986bb169ae","slug":"rat-ptpn1","display_name":"Rat protein tyrosine phosphatase 1B / Ptpn1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"8bf791ac-35f7-56c9-b395-f1783efcbf10","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-rat-ptp1b-response-event","event_type":"biochemical_relationship","label":"A protein that can oppose insulin signaling was reduced in this rat model.","description":"Obese rats receiving chromium picolinate had lower muscle PTP1B abundance and lower activity after correction for protein content.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"89d5d39c-2663-5e0c-8eae-562a5b25a47d","slug":"chromium-iii-picolinate","display_name":"Chromium(III) picolinate / CrPic","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"286c37b4-6064-5a17-b556-81986bb169ae","slug":"rat-ptpn1","display_name":"Rat protein tyrosine phosphatase 1B / Ptpn1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chromium-research/16424121.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b0fa1d0917ec9cf4f3d309f2e9eb0e2b498e083ed1ff881b8f8df94a3cbe8119\", \"start_char\": 0, \"end_char\": 1734, \"text_sha256\": \"b0fa1d0917ec9cf4f3d309f2e9eb0e2b498e083ed1ff881b8f8df94a3cbe8119\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Three-month supplementation and acute muscle insulin-signaling experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Chromium picolinate supplying approximately 80 µg Cr/kg/day; small obese and lean treatment groups","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Model-specific supplementation. Lower phosphatase protein and activity do not prove direct chromium binding to PTP1B. Different results from cultured adipocytes are contextual, not a reason to erase either study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chromium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chromium","display_name":"Chromium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Male JCR:LA-cp obese and lean rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A protein that can oppose insulin signaling was reduced in this rat model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chromium-p16424121] Chromium picolinate enhances skeletal muscle cellular insulin signaling in vivo in obese, insulin-resistant JCR:LA-cp rats. (2006). https://pubmed.ncbi.nlm.nih.gov/16424121/ DOI: 10.1093/jn/136.2.415","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Vastus lateralis skeletal muscle","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0e8947ae-411c-5cc9-88ac-7be8b3dc0551","evidence_kind":"source_excerpt","locator":"Lines 445-456","start_line":445,"end_line":456,"excerpt":"### chromium-rat-ptp1b-response\nObese rats receiving chromium picolinate had lower muscle PTP1B abundance and lower activity after correction for protein content.\nCondition category: normal\nnutrient_topic: Chromium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A protein that can oppose insulin signaling was reduced in this rat model.\norganism: Male JCR:LA-cp obese and lean rats\ntissue_or_cell_type: Vastus lateralis skeletal muscle\nexperimental_model: Three-month supplementation and acute muscle insulin-signaling experiment\nlimitations: Model-specific supplementation. Lower phosphatase protein and activity do not prove direct chromium binding to PTP1B. Different results from cultured adipocytes are contextual, not a reason to erase either study.\nexposure: Chromium picolinate supplying approximately 80 µg Cr/kg/day; small obese and lean treatment groups\nevidence_span: {\"source_cache\": \"artifacts/chromium-research/16424121.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b0fa1d0917ec9cf4f3d309f2e9eb0e2b498e083ed1ff881b8f8df94a3cbe8119\", \"start_char\": 0, \"end_char\": 1734, \"text_sha256\": \"b0fa1d0917ec9cf4f3d309f2e9eb0e2b498e083ed1ff881b8f8df94a3cbe8119\"}\n[chromium-p16424121] Chromium picolinate enhances skeletal muscle cellular insulin signaling in vivo in obese, insulin-resistant JCR:LA-cp rats. 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