{"id":"a5c4ac2d-edae-593d-8b51-d946bc7e63c8","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-ggt-nac-rescue","predicate":"restores_in_model","statement":"Oral NAC restored normal growth rates in the Ggt1-null mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4f58844a-d6b6-5a9c-9e45-4edcf4c44393","mechanism_event_label":"Providing an alternative cysteine source bypassed part of the salvage problem.","subject":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"object":{"id":"b3b6bd9c-3fc6-5da4-8a8a-7f7d76d1e8cf","slug":"ggt-null-growth","display_name":"Growth rate of Ggt1-null mice","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4f58844a-d6b6-5a9c-9e45-4edcf4c44393","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-ggt-nac-rescue-event","event_type":"biochemical_relationship","label":"Providing an alternative cysteine source bypassed part of the salvage problem.","description":"Oral NAC restored normal growth rates in the Ggt1-null mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b3b6bd9c-3fc6-5da4-8a8a-7f7d76d1e8cf","slug":"ggt-null-growth","display_name":"Growth rate of Ggt1-null mice","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/glutathione-research/8755578.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"94d2520bc0124611c649d943ab9acd9cdf73cd3cb99d66640338a57bda6f6dfb\", \"start_char\": 0, \"end_char\": 1159, \"text_sha256\": \"94d2520bc0124611c649d943ab9acd9cdf73cd3cb99d66640338a57bda6f6dfb\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Targeted GGT gene disruption and NAC rescue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Ggt1-null versus wild type; oral NAC","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Severe genetic salvage defect; high extracellular GSH did not mean adequate tissue stores.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glutathione research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Providing an alternative cysteine source bypassed part of the salvage problem.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[glutathione-p8755578] Growth retardation and cysteine deficiency in gamma-glutamyl transpeptidase-deficient mice. 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