{"id":"a58c00a3-908d-5c48-b237-2e4b93115b77","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-kinase-route","predicate":"catalyze_formation","statement":"Only four of 38 examined butyrate-producing isolates had detectable butyrate-kinase activity; the alternative terminal pathway includes phosphotransbutyrylase and butyrate kinase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"01f41c19-0245-56b6-8a29-678ab222df90","mechanism_event_label":"A second route existed but was less widespread in this isolate panel.","subject":{"id":"d8496b2c-5b8a-590e-99e1-70bae7e90d84","slug":"bacterial-butyrate-kinases","display_name":"Bacterial butyrate kinases","entity_type_key":"protein_family"},"object":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"01f41c19-0245-56b6-8a29-678ab222df90","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-kinase-route-event","event_type":"observed_relationship","label":"A second route existed but was less widespread in this isolate panel.","description":"Only four of 38 examined butyrate-producing isolates had detectable butyrate-kinase activity; 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the alternative terminal pathway includes phosphotransbutyrylase and butyrate kinase.\nModel: Human-fecal anaerobic isolates, activity assays and PCR.\nLimitations: Do not assign the kinase route to every producer.\nEvidence access: Primary abstract\nRestricted distribution of the butyrate kinase pathway among butyrate-producing bacteria from the human colon. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15028695/ · DOI 10.1128/JB.186.7.2099-2106.2004","model_system":"Human-fecal anaerobic isolates, activity assays and PCR.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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