{"id":"a5668e88-58ba-515a-861e-5fc56a62266d","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-leucine-alpha-camp","predicate":"reduces","statement":"Leucine reduced mouse alpha-cell cAMP even when beta/delta-cell paracrine signaling was limited; cyanide blocked the inhibitory response, supporting mitochondrial-metabolism dependence.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fd3271fd-252c-54b0-949f-60e074c4a22a","mechanism_event_label":"Part of the suppression arose inside the glucagon-producing cell.","subject":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"object":{"id":"f69fbde1-1d11-54da-982c-10b10b6bab60","slug":"mouse-alpha-cell-camp","display_name":"cAMP in mouse pancreatic alpha cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fd3271fd-252c-54b0-949f-60e074c4a22a","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-leucine-alpha-camp-event","event_type":"observed_relationship","label":"Part of the suppression arose inside the glucagon-producing cell.","description":"Leucine reduced mouse alpha-cell cAMP even when beta/delta-cell paracrine signaling was limited; 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paracrine blockers and metabolic inhibitors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cyanide is broad; the experiment does not identify one unique mitochondrial enzyme as the leucine target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Part of the suppression arose inside the glucagon-producing cell.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Leucine Suppresses α-Cell cAMP and Glucagon Secretion via a Combination of Cell-Intrinsic and Islet Paracrine Signaling. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38870025/ · DOI 10.2337/db23-1013","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"67954f28-e40e-5c36-8804-8b52b8c4f0a2","evidence_kind":"source_excerpt","locator":"Lines 352-358","start_line":352,"end_line":358,"excerpt":"## alanine-leucine-alpha-camp\nPart of the suppression arose inside the glucagon-producing cell.\nLeucine reduced mouse alpha-cell cAMP even when beta/delta-cell paracrine signaling was limited; cyanide blocked the inhibitory response, supporting mitochondrial-metabolism dependence.\nModel: Mouse islet and dispersed-cell experiments; paracrine blockers and metabolic inhibitors.\nLimitations: Cyanide is broad; the experiment does not identify one unique mitochondrial enzyme as the leucine target.\nEvidence access: Primary abstract\nLeucine Suppresses α-Cell cAMP and Glucagon Secretion via a Combination of Cell-Intrinsic and Islet Paracrine Signaling. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38870025/ · DOI 10.2337/db23-1013","model_system":"Mouse islet and dispersed-cell experiments; paracrine blockers and metabolic inhibitors.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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