{"id":"a562c200-60af-5ef7-a958-b955e7659693","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-gpr158-binding","predicate":"binds","statement":"Radioligand binding and mutagenesis supported direct glycine recognition by the GPR158 Cache domain, with an estimated binding Kd near 3 micromolar in receptor-expressing membranes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"567bf6fa-386e-51b8-a068-93f7031e62a2","mechanism_event_label":"Glycine has a receptor beyond its familiar chloride channel and NMDA site.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"c1793375-e328-5a81-940b-046c14ad45fe","slug":"gpr158-recombinant-assay","display_name":"Recombinant GPR158 in the 2023 binding/signaling assays","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"567bf6fa-386e-51b8-a068-93f7031e62a2","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-gpr158-binding-event","event_type":"observed_relationship","label":"Glycine has a receptor beyond its familiar chloride channel and NMDA site.","description":"Radioligand binding and mutagenesis supported direct glycine recognition by the GPR158 Cache domain, with an estimated binding Kd near 3 micromolar in receptor-expressing membranes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c1793375-e328-5a81-940b-046c14ad45fe","slug":"gpr158-recombinant-assay","display_name":"Recombinant GPR158 in the 2023 binding/signaling assays","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant GPR158 in HEK293-derived membrane binding assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Construct species is not resolved by the accessed main-text methods; human host cells do not establish human receptor sequence. Binding Kd is not an oral dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Glycine has a receptor beyond its familiar chloride channel and NMDA site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orphan receptor GPR158 serves as a metabotropic glycine receptor: mGlyR. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36996198/ · DOI 10.1126/science.add7150","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f964f16e-fd5e-5aa9-a25c-58e26ba91ba2","evidence_kind":"source_excerpt","locator":"Lines 218-224","start_line":218,"end_line":224,"excerpt":"## glycine-gpr158-binding\nGlycine has a receptor beyond its familiar chloride channel and NMDA site.\nRadioligand binding and mutagenesis supported direct glycine recognition by the GPR158 Cache domain, with an estimated binding Kd near 3 micromolar in receptor-expressing membranes.\nModel: Recombinant GPR158 in HEK293-derived membrane binding assays.\nLimitations: Construct species is not resolved by the accessed main-text methods; human host cells do not establish human receptor sequence. Binding Kd is not an oral dose.\nEvidence access: Primary full text\nOrphan receptor GPR158 serves as a metabotropic glycine receptor: mGlyR. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36996198/ · DOI 10.1126/science.add7150","model_system":"Recombinant GPR158 in HEK293-derived membrane binding assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4fabd2fc8e71ca156c91023e7340be3f5a02ae03e91d3a24b231c2adfb0de0aa","revision_id":"24801e49-34a8-53c3-bdf5-b59143441450","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}