{"id":"a5059689-472b-532a-96f0-1bb2cc302112","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-podxl","predicate":"increases_abundance_of","statement":"PODXL upregulation was confirmed by immunoblotting.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5bffca15-7536-5849-8c69-f3ac0e11372c","mechanism_event_label":"A cell-surface protein changed after exposure.","subject":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"object":{"id":"d155a31f-e898-5e45-bc7e-aebad80ed026","slug":"podxl","display_name":"Human podocalyxin / PODXL","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"5bffca15-7536-5849-8c69-f3ac0e11372c","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-podxl-event","event_type":"biochemical_relationship","label":"A cell-surface protein changed after exposure.","description":"PODXL upregulation was confirmed by immunoblotting.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d155a31f-e898-5e45-bc7e-aebad80ed026","slug":"podxl","display_name":"Human podocalyxin / PODXL","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chlorogenic_acid-research/36076497.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"70d2217b479c3b8e299fd8b94dda90a12086be9c8ed756ad3359dc4272285a0a\", \"start_char\": 0, \"end_char\": 1645, \"text_sha256\": \"70d2217b479c3b8e299fd8b94dda90a12086be9c8ed756ad3359dc4272285a0a\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Quantitative proteomics, immunoblots and endothelial functional assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"5 micromolar CGA for 24 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An immortalized cell model, not intact human vessels. Enrichment of PI3K/AKT and Rap1 pathways is hypothesis generation; increased angiogenesis is not universally desirable.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human EA.hy926 cell line","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A cell-surface protein changed after exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chlorogenic_acid-p36076497] Chlorogenic acid enhances endothelial barrier function and promotes endothelial tube formation: A proteomics approach and functional validation. 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Enrichment of PI3K/AKT and Rap1 pathways is hypothesis generation; increased angiogenesis is not universally desirable.\nexposure: 5 micromolar CGA for 24 hours\nevidence_span: {\"source_cache\": \"artifacts/chlorogenic_acid-research/36076497.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"70d2217b479c3b8e299fd8b94dda90a12086be9c8ed756ad3359dc4272285a0a\", \"start_char\": 0, \"end_char\": 1645, \"text_sha256\": \"70d2217b479c3b8e299fd8b94dda90a12086be9c8ed756ad3359dc4272285a0a\"}\n[chlorogenic_acid-p36076497] Chlorogenic acid enhances endothelial barrier function and promotes endothelial tube formation: A proteomics approach and functional validation. 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