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(2014). https://pubmed.ncbi.nlm.nih.gov/24580380/ DOI: 10.1021/bi500101z","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified enzyme–primer–template system","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"648d63e2-5af6-5c12-b42b-3346a1f5f974","evidence_kind":"source_excerpt","locator":"Lines 685-696","start_line":685,"end_line":696,"excerpt":"### phosphorus-dna-phosphate-chemistry\nChanging pyrophosphate-mimicking nucleotide leaving groups altered POLB chemical-step kinetics in a base-pair-dependent manner.\nCondition category: normal\nnutrient_topic: Phosphorus research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The nucleotide’s phosphate-chain chemistry is part of DNA synthesis fidelity, not merely structural packaging.\norganism: Purified DNA polymerase beta; construct species unresolved in the inspected text\ntissue_or_cell_type: Purified enzyme–primer–template system\nexperimental_model: dNTP leaving-group analogue kinetics\nlimitations: Chemistry with designed nucleotide analogues, not a measurement of dietary phosphorus and DNA repair; no free-phosphate-to-DNA shortcut is asserted.\nexposure: Beta/gamma pyrophosphate-mimicking dNTP analogues across matched and mismatched base pairs\nevidence_span: {\"source_cache\": \"artifacts/phosphorus-research/24580380.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7ffa6a3c6ccf9f3a9d52240080160bfcf00d832210168f91b7ac0b9a39be30a2\", \"start_char\": 0, \"end_char\": 1081, \"text_sha256\": \"7ffa6a3c6ccf9f3a9d52240080160bfcf00d832210168f91b7ac0b9a39be30a2\"}\n[phosphorus-p24580380] Transition state in DNA polymerase β catalysis: rate-limiting chemistry altered by base-pair configuration. 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