{"id":"a4d3d75b-6930-57d9-908e-88a271254af0","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-bone-wnt-loss","predicate":"cd8_cell_loss_blocks","statement":"Reconstituting TCR-beta-deficient mice with Wnt10b-deficient CD8 T cells prevented butyrate-induced bone formation and mass acquisition; lowering Treg numbers also prevented the response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"39dff442-33ec-5241-a3d4-b4b4947bf282","mechanism_event_label":"Butyrate could not replace the missing immune-cell signal.","subject":{"id":"24f2ec5a-b6dd-5e40-bd54-b5650827ef07","slug":"mouse-wnt10b","display_name":"Mouse Wnt family member 10B / Wnt10b","entity_type_key":"protein"},"object":{"id":"e408fd51-36b5-565a-be99-a532b9aec9c7","slug":"mouse-butyrate-bone-formation","display_name":"Mouse bone formation after butyrate","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"39dff442-33ec-5241-a3d4-b4b4947bf282","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-bone-wnt-loss-event","event_type":"observed_relationship","label":"Butyrate could not replace the missing immune-cell signal.","description":"Reconstituting TCR-beta-deficient mice with Wnt10b-deficient CD8 T cells prevented butyrate-induced bone formation and mass acquisition; lowering Treg numbers also prevented the response.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"24f2ec5a-b6dd-5e40-bd54-b5650827ef07","slug":"mouse-wnt10b","display_name":"Mouse Wnt family member 10B / Wnt10b","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e408fd51-36b5-565a-be99-a532b9aec9c7","slug":"mouse-butyrate-bone-formation","display_name":"Mouse bone formation after butyrate","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse cellular reconstitution and Treg reduction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A genetic dependency is not evidence that a person needs more dietary calcium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Butyrate could not replace the missing immune-cell signal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30446387/ · DOI 10.1016/j.immuni.2018.10.013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"fb6579e3-a63d-5c58-a1b2-2ebf587a90e0","evidence_kind":"source_excerpt","locator":"Lines 470-476","start_line":470,"end_line":476,"excerpt":"## butyrate-bone-wnt-loss\nButyrate could not replace the missing immune-cell signal.\nReconstituting TCR-beta-deficient mice with Wnt10b-deficient CD8 T cells prevented butyrate-induced bone formation and mass acquisition; lowering Treg numbers also prevented the response.\nModel: Mouse cellular reconstitution and Treg reduction.\nLimitations: A genetic dependency is not evidence that a person needs more dietary calcium.\nEvidence access: Primary abstract\nThe Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30446387/ · DOI 10.1016/j.immuni.2018.10.013","model_system":"Mouse cellular reconstitution and Treg reduction.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"a1253eb4426fc45d7b7c113028aa4ba2e9f89a9653272f3795437fc222bad558","revision_id":"c2320e65-a252-57a9-8838-7abf3d23400e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}