{"id":"a4685a1c-3dd3-554d-adae-477af20eb929","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-albumin-sulfate","predicate":"binds_more_stably_than_parent","statement":"Luteolin sulfate formed a more stable albumin complex than parent luteolin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8b5eac42-e8b4-5ea4-b261-665678303758","mechanism_event_label":"Conjugation changes carrier binding.","subject":{"id":"64d39ecb-18b8-5f67-8514-501180eccc69","slug":"luteolin-3-prime-o-sulfate","display_name":"Luteolin 3′-O-sulfate","entity_type_key":"small_molecule"},"object":{"id":"d74adc7c-c253-5604-99bf-73d5ed0c0701","slug":"human-serum-albumin","display_name":"Human serum albumin","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"8b5eac42-e8b4-5ea4-b261-665678303758","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-albumin-sulfate-event","event_type":"observed_relationship","label":"Conjugation changes carrier binding.","description":"Luteolin sulfate formed a more stable albumin complex than parent luteolin.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"64d39ecb-18b8-5f67-8514-501180eccc69","slug":"luteolin-3-prime-o-sulfate","display_name":"Luteolin 3′-O-sulfate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d74adc7c-c253-5604-99bf-73d5ed0c0701","slug":"human-serum-albumin","display_name":"Human serum albumin","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human serum-albumin binding assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Binding alone does not establish displacement toxicity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Conjugation changes carrier binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Interaction of luteolin, naringenin, and their sulfate and glucuronide conjugates with human serum albumin, cytochrome P450 (CYP2C9, CYP2C19, and CYP3A4) enzymes and organic anion transporting polypeptide (OATP1B1 and OATP2B1) transporters. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36481402/ · DOI 10.1016/j.biopha.2022.114078","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1c266c33-35ee-52e9-bd6c-9c35f416fc50","evidence_kind":"source_excerpt","locator":"Lines 236-242","start_line":236,"end_line":242,"excerpt":"## luteolin-albumin-sulfate\nConjugation changes carrier binding.\nLuteolin sulfate formed a more stable albumin complex than parent luteolin.\nModel: Human serum-albumin binding assay.\nLimitations: Binding alone does not establish displacement toxicity.\nEvidence access: Primary abstract\nInteraction of luteolin, naringenin, and their sulfate and glucuronide conjugates with human serum albumin, cytochrome P450 (CYP2C9, CYP2C19, and CYP3A4) enzymes and organic anion transporting polypeptide (OATP1B1 and OATP2B1) transporters. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36481402/ · DOI 10.1016/j.biopha.2022.114078","model_system":"Human serum-albumin binding assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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