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(2000). https://pubmed.ncbi.nlm.nih.gov/10722724/ DOI: 10.1074/jbc.275.12.8794","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Serotonin acetylation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"16668569-d4c3-5150-a46a-4e69b6f1db73","evidence_kind":"source_excerpt","locator":"Lines 175-186","start_line":175,"end_line":186,"excerpt":"### melatonin-aanat-serotonin\nHuman AANAT transferred an acetyl group from acetyl-CoA to serotonin, producing N-acetylserotonin.\nCondition category: normal\nnutrient_topic: Melatonin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Serotonin is modified before it becomes melatonin.\norganism: Human AANAT expressed in bacteria\ntissue_or_cell_type: Serotonin acetylation\nexperimental_model: Purified recombinant enzyme substrate-specificity study\nlimitations: Biochemical mechanism, not evidence that dietary B5 or acetyl-CoA availability limits melatonin production in all humans.\nexposure: Serotonin and radiolabeled acetyl-CoA; product HPLC\nevidence_span: {\"source_cache\": \"artifacts/melatonin-research/10722724.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7fb8418b17e92c5b3fed5d9c08afdb32f28c922dfe94274d3bb02b7e12b2b8c8\", \"start_char\": 0, \"end_char\": 1857, \"text_sha256\": \"7fb8418b17e92c5b3fed5d9c08afdb32f28c922dfe94274d3bb02b7e12b2b8c8\"}\n[melatonin-p10722724] Substrate specificity and inhibition studies of human serotonin N-acetyltransferase. 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