{"id":"a42e63bb-c93a-593d-9938-aedd8fa64086","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-tht","predicate":"interferes_with","statement":"Both oxidized and unoxidized EGCG bound fibrils and prevented Thioflavin T binding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f2881f6b-6f40-5750-ba23-f0f724231c0d","mechanism_event_label":"A lower dye signal alone can be mistaken for less amyloid.","subject":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"object":{"id":"9f582902-acb6-5c69-abc2-96e5a87cb2aa","slug":"thioflavin-t-fibril-signal","display_name":"Thioflavin T fibril-binding signal","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f2881f6b-6f40-5750-ba23-f0f724231c0d","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-tht-event","event_type":"observed_relationship","label":"A lower dye signal alone can be mistaken for less amyloid.","description":"Both oxidized and unoxidized EGCG bound fibrils and prevented Thioflavin T binding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9f582902-acb6-5c69-abc2-96e5a87cb2aa","slug":"thioflavin-t-fibril-signal","display_name":"Thioflavin T fibril-binding signal","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e66ddcc-1636-5054-b366-703d8ea568ba","slug":"oxidized-egcg-pool","display_name":"Oxidized EGCG reaction products, unresolved mixture","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ea6e541b-f908-5022-9958-ba12d8c0a5f9","slug":"thioflavin-t","display_name":"Thioflavin T","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Fibril binding/assay-interference experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Use independent structural and toxicity measurements to distinguish remodeling from dye displacement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A lower dye signal alone can be mistaken for less amyloid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Toward the molecular mechanism(s) by which EGCG treatment remodels mature amyloid fibrils. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23611538/ · DOI 10.1021/ja3115696","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0d6c8358-f75a-5f05-ad27-44e67a8e9774","evidence_kind":"source_excerpt","locator":"Lines 388-394","start_line":388,"end_line":394,"excerpt":"## egcg-tht\nA lower dye signal alone can be mistaken for less amyloid.\nBoth oxidized and unoxidized EGCG bound fibrils and prevented Thioflavin T binding.\nModel: Fibril binding/assay-interference experiments.\nLimitations: Use independent structural and toxicity measurements to distinguish remodeling from dye displacement.\nEvidence access: primary abstract.\nToward the molecular mechanism(s) by which EGCG treatment remodels mature amyloid fibrils. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23611538/ · DOI 10.1021/ja3115696","model_system":"Fibril binding/assay-interference experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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