{"id":"a4226491-4102-569b-8bc6-e862ced96158","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-slc22a4-not-carnitine","predicate":"does_not_transport","statement":"Wild-type human SLC22A4 did not mediate carnitine transport in the comparative HEK293 expression assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"944c68e9-f16e-5088-8456-79a65a2301c6","mechanism_event_label":"The historical carrier name does not mean carnitine uses this route.","subject":{"id":"1509c925-fc5e-5a36-aa59-96bcc489965a","slug":"slc22a4","display_name":"Human ergothioneine transporter OCTN1 / SLC22A4","entity_type_key":"protein"},"object":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"944c68e9-f16e-5088-8456-79a65a2301c6","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-slc22a4-not-carnitine-event","event_type":"observed_relationship","label":"The historical carrier name does not mean carnitine uses this route.","description":"Wild-type human SLC22A4 did not mediate carnitine transport in the comparative HEK293 expression assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1509c925-fc5e-5a36-aa59-96bcc489965a","slug":"slc22a4","display_name":"Human ergothioneine transporter OCTN1 / SLC22A4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Wild-type human SLC22A4/SLC22A5 transport comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay-specific discrimination; engineered mutants have different properties.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The historical carrier name does not mean carnitine uses this route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Substrate discrimination by ergothioneine transporter SLC22A4 and carnitine transporter SLC22A5: gain-of-function by interchange of selected amino acids. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19814996/ · DOI 10.1016/j.bbamem.2009.09.019","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4922888c-b9c6-5b1c-97f6-6cf2b0917134","evidence_kind":"source_excerpt","locator":"Lines 32-38","start_line":32,"end_line":38,"excerpt":"## ergothioneine-slc22a4-not-carnitine\nThe historical carrier name does not mean carnitine uses this route.\nWild-type human SLC22A4 did not mediate carnitine transport in the comparative HEK293 expression assay.\nModel: Wild-type human SLC22A4/SLC22A5 transport comparison.\nLimitations: Assay-specific discrimination; engineered mutants have different properties.\nEvidence access: Primary abstract\nSubstrate discrimination by ergothioneine transporter SLC22A4 and carnitine transporter SLC22A5: gain-of-function by interchange of selected amino acids. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19814996/ · DOI 10.1016/j.bbamem.2009.09.019","model_system":"Wild-type human SLC22A4/SLC22A5 transport comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}