{"id":"a3efb13f-a7a0-50c7-9275-fea8135c5bbe","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-depletion-increases-mitochondrial-ammonia","predicate":"depletion-increases","statement":"Renal mitochondria from potassium-depleted rats produced more ammonia during glutamine-metabolism assays after three days of potassium-free feeding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"24bfe3af-dfb0-5a39-beff-28368a92d234","mechanism_event_label":"The kidney adaptation included greater ammonia production, beyond changes in enzyme abundance.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"59404da3-8285-57a2-a3cc-4cb35f13a7b0","slug":"renal-ammonia-production","display_name":"Renal ammonia production","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"24bfe3af-dfb0-5a39-beff-28368a92d234","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-depletion-increases-mitochondrial-ammonia-event","event_type":"biochemical_relationship","label":"The kidney adaptation included greater ammonia production, beyond changes in enzyme abundance.","description":"Renal mitochondria from potassium-depleted rats produced more ammonia during glutamine-metabolism assays after three days of potassium-free feeding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"role":"upstream","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"59404da3-8285-57a2-a3cc-4cb35f13a7b0","slug":"renal-ammonia-production","display_name":"Renal ammonia production","entity_type_key":"cellular_process"},"role":"downstream","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1374ec24-1a5b-552b-982f-51f9082f4187","slug":"ammonia","display_name":"Ammonia","entity_type_key":"small_molecule"},"role":"reported-product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e923077b-9786-5ab9-b23b-638777ac6a85","slug":"ammonium-ion","display_name":"Ammonium ion","entity_type_key":"ion"},"role":"equilibrium-species","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"9c85ad91-9054-59c3-aca4-eeade9c24387","slug":"gls","display_name":"Kidney-type glutaminase / GLS","entity_type_key":"protein"},"role":"pathway-enzyme","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Potassium depletion changes mitochondrial glutamine nitrogen metabolism; enzyme capacity and substrate entry need separate accounting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"Renal mitochondria from potassium-depleted rats produced more ammonia during glutamine-metabolism assays after three days of potassium-free feeding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Rats on potassium-free diet with renal mitochondrial and cortical-tubule ammonia assays during depletion and potassium refeeding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rats on potassium-free diet with renal mitochondrial and cortical-tubule ammonia assays during depletion and potassium refeeding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Isolated mitochondrial assay, not whole-body bicarbonate balance. Glutaminase activity rose earlier than ammonia flux; mitochondrial glutamine entry was proposed as a limiting step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The kidney adaptation included greater ammonia production, beyond changes in enzyme abundance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sastrasinh-1986-mitochondrial-ammonia] Renal mitochondrial glutamine metabolism during K+ depletion (1986). https://pubmed.ncbi.nlm.nih.gov/3963205/ DOI: 10.1152/ajprenal.1986.250.4.F667","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"renal mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"073b79d2-7077-59ef-b4a7-6975469121fe","evidence_kind":"source_excerpt","locator":"Lines 1041-1053","start_line":1041,"end_line":1053,"excerpt":"### k-depletion-increases-mitochondrial-ammonia\nRenal mitochondria from potassium-depleted rats produced more ammonia during glutamine-metabolism assays after three days of potassium-free feeding.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The kidney adaptation included greater ammonia production, beyond changes in enzyme abundance.\norganism: Rattus norvegicus\ntissue_or_cell_type: renal mitochondria\nexperimental_model: Rats on potassium-free diet with renal mitochondrial and cortical-tubule ammonia assays during depletion and potassium refeeding.\nlimitations: Isolated mitochondrial assay, not whole-body bicarbonate balance. Glutaminase activity rose earlier than ammonia flux; mitochondrial glutamine entry was proposed as a limiting step.\ncross_nutrient: Potassium depletion changes mitochondrial glutamine nitrogen metabolism; enzyme capacity and substrate entry need separate accounting.\nexperimental-exposure: Rats on potassium-free diet with renal mitochondrial and cortical-tubule ammonia assays during depletion and potassium refeeding.\nendpoint: Renal mitochondria from potassium-depleted rats produced more ammonia during glutamine-metabolism assays after three days of potassium-free feeding.\n[sastrasinh-1986-mitochondrial-ammonia] Renal mitochondrial glutamine metabolism during K+ depletion (1986). https://pubmed.ncbi.nlm.nih.gov/3963205/ DOI: 10.1152/ajprenal.1986.250.4.F667","model_system":"Rats on potassium-free diet with renal mitochondrial and cortical-tubule ammonia assays during depletion and potassium refeeding.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sastrasinh-1986-mitochondrial-ammonia] Renal mitochondrial glutamine metabolism during K+ depletion (1986). https://pubmed.ncbi.nlm.nih.gov/3963205/ DOI: 10.1152/ajprenal.1986.250.4.F667","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}