{"id":"a2d3fbdc-be2d-58c1-914d-dffc0be82cfe","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-durrant-direct-contrast","predicate":"has-largely-null-placebo-adjusted-probe-contrasts","statement":"Difference-in-difference testing found no significant probe changes in the white-European cohort or pooled D2/D3-versus-placebo analyses; five were found specifically for D3 versus placebo in the South-Asian cohort, largely driven by placebo-group changes.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d53fb0f0-be34-580e-83ca-b74d9e5734e8","mechanism_event_label":"The strongest direct comparisons were much less conclusive than the within-group gene-change lists.","subject":{"id":"1e377530-26da-59d9-99e7-3b15f86ea2b9","slug":"d2-d3-equal-dose-comparison","display_name":"Matched-dose D2 versus D3 comparison","entity_type_key":"cellular_process"},"object":{"id":"dd4b6fa5-307f-5b2d-aa23-afe5abde30bb","slug":"blood-transcriptome-treatment-contrast","display_name":"Placebo-adjusted whole-blood transcriptome contrast","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d53fb0f0-be34-580e-83ca-b74d9e5734e8","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-durrant-direct-contrast-event","event_type":"observed_intervention","label":"The strongest direct comparisons were much less conclusive than the within-group gene-change lists.","description":"Difference-in-difference testing found no significant probe changes in the white-European cohort or pooled D2/D3-versus-placebo analyses; five were found specifically for D3 versus placebo in the South-Asian cohort, largely driven by placebo-group changes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6e1cf31c-f78e-596b-b1b3-70585cf9c948","slug":"ergocalciferol","display_name":"Ergocalciferol / vitamin D2","entity_type_key":"small_molecule"},"role":"intervention","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b162e431-b442-5400-9335-ec91f7875a4e","slug":"cholecalciferol","display_name":"Cholecalciferol","entity_type_key":"small_molecule"},"role":"intervention","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1e377530-26da-59d9-99e7-3b15f86ea2b9","slug":"d2-d3-equal-dose-comparison","display_name":"Matched-dose D2 versus D3 comparison","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"dd4b6fa5-307f-5b2d-aa23-afe5abde30bb","slug":"blood-transcriptome-treatment-contrast","display_name":"Placebo-adjusted whole-blood transcriptome contrast","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"97 participant transcriptomic subset of Tripkovic 2017; 67 white-European and 30 South-Asian women","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"15 micrograms/day D2 or D3 or placebo for 12 winter weeks; blood microarrays at baseline/end.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Exploratory selected trial subset; differences in baseline status, season and whole-blood cell composition may matter. Within-group significance is not a significant difference between groups; no infection endpoint.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The strongest direct comparisons were much less conclusive than the within-group gene-change lists.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[durrant2022] Vitamins D2 and D3 Have Overlapping But Different Effects on the Human Immune System Revealed Through Analysis of the Blood Transcriptome. (2022). https://pubmed.ncbi.nlm.nih.gov/35281034/ DOI: 10.3389/fimmu.2022.790444","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Whole blood","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"75e25e86-e12f-5ac6-aafe-d52882038c68","evidence_kind":"source_excerpt","locator":"Lines 1087-1098","start_line":1087,"end_line":1098,"excerpt":"### vd-durrant-direct-contrast\nDifference-in-difference testing found no significant probe changes in the white-European cohort or pooled D2/D3-versus-placebo analyses; five were found specifically for D3 versus placebo in the South-Asian cohort, largely driven by placebo-group changes.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The strongest direct comparisons were much less conclusive than the within-group gene-change lists.\norganism: Homo sapiens\ntissue_or_cell_type: Whole blood\nexperimental_model: 97 participant transcriptomic subset of Tripkovic 2017; 67 white-European and 30 South-Asian women\nlimitations: Exploratory selected trial subset; differences in baseline status, season and whole-blood cell composition may matter. Within-group significance is not a significant difference between groups; no infection endpoint.\nexposure: 15 micrograms/day D2 or D3 or placebo for 12 winter weeks; blood microarrays at baseline/end.\ncross_nutrient: false\n[durrant2022] Vitamins D2 and D3 Have Overlapping But Different Effects on the Human Immune System Revealed Through Analysis of the Blood Transcriptome. (2022). https://pubmed.ncbi.nlm.nih.gov/35281034/ DOI: 10.3389/fimmu.2022.790444","model_system":"97 participant transcriptomic subset of Tripkovic 2017; 67 white-European and 30 South-Asian women","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [durrant2022] Vitamins D2 and D3 Have Overlapping But Different Effects on the Human Immune System Revealed Through Analysis of the Blood Transcriptome. 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