{"id":"a2d1c8ed-f120-59d9-8a52-a4bc0be6c20b","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-defined-synuclein","predicate":"reduces_coculture_aggregation","statement":"The reagent reduced alpha-synuclein aggregation signals in co-cultures; proteinase-K resistance was not significantly reduced in transfected-only cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"116addea-94df-583e-8e14-cfdd8a781f26","mechanism_event_label":"The effect depended on how aggregates were produced and measured.","subject":{"id":"d6140c5e-730e-5710-b502-424b7b4f6836","slug":"fungal-fulvic-acid-479-66-3","display_name":"Defined fungal fulvic acid / CAS 479-66-3","entity_type_key":"small_molecule"},"object":{"id":"2ac79b6b-5333-55da-a444-c3b09ec8727c","slug":"human-hek293-asyn-aggregation","display_name":"Alpha-synuclein aggregation in human HEK293 co-cultures","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"116addea-94df-583e-8e14-cfdd8a781f26","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-defined-synuclein-event","event_type":"observed_relationship","label":"The effect depended on how aggregates were produced and measured.","description":"The reagent reduced alpha-synuclein aggregation signals in co-cultures; proteinase-K resistance was not significantly reduced in transfected-only cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d6140c5e-730e-5710-b502-424b7b4f6836","slug":"fungal-fulvic-acid-479-66-3","display_name":"Defined fungal fulvic acid / CAS 479-66-3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2ac79b6b-5333-55da-a444-c3b09ec8727c","slug":"human-hek293-asyn-aggregation","display_name":"Alpha-synuclein aggregation in human HEK293 co-cultures","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"310ed26c-0027-5fc6-91f7-91182f559a19","slug":"alpha-synuclein-fibrils","display_name":"Alpha-synuclein fibrils","entity_type_key":"protein_state"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"2020 study; Cayman CAS 479-66-3 at 37 micromolar; HEK293 constructs or purified K18 tau as specified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The methods identify a defined fungal compound, not an interchangeable environmental mixture. Supplier identity: https://www.caymanchem.com/product/19063/fulvic-acid . No clinical efficacy or CNS exposure was measured. Disassembly of all pre-existing alpha-synuclein aggregates was not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The effect depended on how aggregates were produced and measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Effects of pharmacological modulators of α-synuclein and tau aggregation and internalization. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32732936/ · DOI 10.1038/s41598-020-69744-y","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"19f2fe5b-bf0a-5ddc-99fa-3d844c0b010b","evidence_kind":"source_excerpt","locator":"Lines 180-186","start_line":180,"end_line":186,"excerpt":"## fulvic-acid-defined-synuclein\nThe effect depended on how aggregates were produced and measured.\nThe reagent reduced alpha-synuclein aggregation signals in co-cultures; proteinase-K resistance was not significantly reduced in transfected-only cells.\nModel: 2020 study; Cayman CAS 479-66-3 at 37 micromolar; HEK293 constructs or purified K18 tau as specified.\nLimitations: The methods identify a defined fungal compound, not an interchangeable environmental mixture. Supplier identity: https://www.caymanchem.com/product/19063/fulvic-acid . No clinical efficacy or CNS exposure was measured. Disassembly of all pre-existing alpha-synuclein aggregates was not established.\nEvidence access: Primary full text\nEffects of pharmacological modulators of α-synuclein and tau aggregation and internalization. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32732936/ · DOI 10.1038/s41598-020-69744-y","model_system":"2020 study; Cayman CAS 479-66-3 at 37 micromolar; HEK293 constructs or purified K18 tau as specified.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"52c2c3ee6bb23d943a40e1c39dd9de5cca7acd6efa72479cb824149e85cc7968","revision_id":"94dfc01b-c93e-55ff-8f48-bb2e9a1979f6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}