{"id":"a2986cad-6a9d-5a13-87e9-6e2cb7cdf639","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-host-conjugation","predicate":"is_conjugated_with_glutamine_to","statement":"Host conjugation of microbially derived phenylacetate with glutamine produces PAGln, the predominant human conjugate in the study; glycine conjugation produces PAGly, predominant in mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cde8b0e4-96f5-598e-80ef-d676124fcd04","mechanism_event_label":"Host amino acids help turn a bacterial product into circulating metabolites.","subject":{"id":"15dd7892-f298-50d1-aaef-7f7b9f94a033","slug":"phenylacetic-acid","display_name":"Phenylacetic acid / phenylacetate","entity_type_key":"small_molecule"},"object":{"id":"8968a92b-40e9-56fe-8d76-d8776750dbd6","slug":"phenylacetylglutamine","display_name":"Phenylacetylglutamine / PAGln","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"cde8b0e4-96f5-598e-80ef-d676124fcd04","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-host-conjugation-event","event_type":"observed_relationship","label":"Host amino acids help turn a bacterial product into circulating metabolites.","description":"Host conjugation of microbially derived phenylacetate with glutamine produces PAGln, the predominant human conjugate in the study; glycine conjugation produces PAGly, predominant in mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15dd7892-f298-50d1-aaef-7f7b9f94a033","slug":"phenylacetic-acid","display_name":"Phenylacetic acid / phenylacetate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8968a92b-40e9-56fe-8d76-d8776750dbd6","slug":"phenylacetylglutamine","display_name":"Phenylacetylglutamine / PAGln","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"a694e365-dec9-5fe5-9098-f9eb19928c8d","slug":"phenylacetylglycine","display_name":"Phenylacetylglycine / PAGly","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human/mouse metabolomics study; established conjugation route described in primary full text.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is not a purified conjugating-enzyme kinetic experiment and does not show clinically meaningful glutamine or glycine depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Host amino acids help turn a bacterial product into circulating metabolites.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic Receptors. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32142679/ · DOI 10.1016/j.cell.2020.02.016","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2c32b2c4-ec0e-5328-ae43-6945d7580b8e","evidence_kind":"source_excerpt","locator":"Lines 358-364","start_line":358,"end_line":364,"excerpt":"## l-phenylalanine-host-conjugation\nHost amino acids help turn a bacterial product into circulating metabolites.\nHost conjugation of microbially derived phenylacetate with glutamine produces PAGln, the predominant human conjugate in the study; glycine conjugation produces PAGly, predominant in mice.\nModel: Human/mouse metabolomics study; established conjugation route described in primary full text.\nLimitations: This is not a purified conjugating-enzyme kinetic experiment and does not show clinically meaningful glutamine or glycine depletion.\nEvidence access: Primary full text\nA Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic Receptors. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32142679/ · DOI 10.1016/j.cell.2020.02.016","model_system":"Human/mouse metabolomics study; established conjugation route described in primary full text.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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