{"id":"a287e73c-0626-5ad2-ab6b-72434dc12674","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-gly-colgalt-gt1-stability","predicate":"supports","statement":"Calcium and UDP-galactose bound in COLGALT1’s noncatalytic GT1 domain contribute to folding stability.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"144e6be1-ddd2-56ab-b954-e836d650fdc6","mechanism_event_label":"Calcium and the donor sugar also help stabilize a separate part of this manganese enzyme.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"a4c725ed-d652-5c40-bf10-ec12db6e55f5","slug":"colgalt1-folding-stability","display_name":"COLGALT1 folding stability","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"144e6be1-ddd2-56ab-b954-e836d650fdc6","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-gly-colgalt-gt1-stability-event","event_type":"biochemical_relationship","label":"Calcium and the donor sugar also help stabilize a separate part of this manganese enzyme.","description":"Calcium and UDP-galactose bound in COLGALT1’s noncatalytic GT1 domain contribute to folding stability.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4dbb65a6-60cb-5fad-b3eb-73ee7038381d","slug":"colgalt1","display_name":"COLGALT1","entity_type_key":"protein"},"role":"affected_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a76bbd5b-08c9-5d74-8b6f-d69502ffa655","slug":"udp-galactose","display_name":"UDP-galactose","entity_type_key":"small_molecule"},"role":"stabilizing_ligand","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"distinct_catalytic_ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"a4c725ed-d652-5c40-bf10-ec12db6e55f5","slug":"colgalt1-folding-stability","display_name":"COLGALT1 folding stability","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"COLGALT1 (affected_protein); UDP-galactose (stabilizing_ligand); Manganese(II) ion (distinct_catalytic_ion)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/manganese-glycosylation-sources/colgalt2025.abstract.txt\", \"locator\": \"Indexed primary abstract\", \"file_sha256\": \"5a42e5928c56f4e312768d79c9a5601b6f9e76a44bcdd9ede4bc8ea0c8985b60\", \"start_char\": 0, \"end_char\": 1331, \"text_sha256\": \"5a42e5928c56f4e312768d79c9a5601b6f9e76a44bcdd9ede4bc8ea0c8985b60\", \"text_characters\": 1331}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human COLGALT1 structural and biochemical assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Metal substitution and structural-domain comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Modification of collagen-bound hydroxylysine does not establish that extra dietary manganese or free lysine improves collagen in a person.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Manganese research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Calcium and the donor sugar also help stabilize a separate part of this manganese enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mn-gly-colgalt2025] Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1. (2025). https://pubmed.ncbi.nlm.nih.gov/40240392/ DOI: 10.1038/s41467-025-59017-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified protein and collagen peptides","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0f899550-d2b0-5e83-b86f-7c4589c2c64b","evidence_kind":"source_excerpt","locator":"Lines 922-934","start_line":922,"end_line":934,"excerpt":"### mn-gly-colgalt-gt1-stability\nCalcium and UDP-galactose bound in COLGALT1’s noncatalytic GT1 domain contribute to folding stability.\nCondition category: normal\nnutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Calcium and the donor sugar also help stabilize a separate part of this manganese enzyme.\norganism: Homo sapiens\ntissue_or_cell_type: Purified protein and collagen peptides\nexperimental_model: Human COLGALT1 structural and biochemical assays\nlimitations: Modification of collagen-bound hydroxylysine does not establish that extra dietary manganese or free lysine improves collagen in a person.\nexposure: Metal substitution and structural-domain comparisons.\ncross_nutrient: COLGALT1 (affected_protein); UDP-galactose (stabilizing_ligand); Manganese(II) ion (distinct_catalytic_ion)\nevidence_span: {\"source_cache\": \"artifacts/manganese-glycosylation-sources/colgalt2025.abstract.txt\", \"locator\": \"Indexed primary abstract\", \"file_sha256\": \"5a42e5928c56f4e312768d79c9a5601b6f9e76a44bcdd9ede4bc8ea0c8985b60\", \"start_char\": 0, \"end_char\": 1331, \"text_sha256\": \"5a42e5928c56f4e312768d79c9a5601b6f9e76a44bcdd9ede4bc8ea0c8985b60\", \"text_characters\": 1331}\n[mn-gly-colgalt2025] Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1. (2025). https://pubmed.ncbi.nlm.nih.gov/40240392/ DOI: 10.1038/s41467-025-59017-5","model_system":"Human COLGALT1 structural and biochemical assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mn-gly-colgalt2025] Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1. (2025). https://pubmed.ncbi.nlm.nih.gov/40240392/ DOI: 10.1038/s41467-025-59017-5","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"03224387-8a73-5b4f-906d-9f0be6625b7f","stable_key":"import-be889add-cec8-500b-be89-676431432a70","title":"Manganese: enzyme cofactors, glycosylation, transport and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f029ee5a1133a4f296047f06a6f0178deb9fd02e8d9707bc285bbe0f4e08fcb5","revision_id":"a77068c1-5a13-5aa9-bbac-d1cff6d34f15","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}