{"id":"a28109a0-4879-5ab7-8899-41e64bdc6330","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-mttp-chylomicron-loss","predicate":"reduces_secretion","statement":"Primary enterocytes from Mttp-deficient mice secreted less alpha-tocopherol with chylomicrons.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a23246c7-1b4b-5c3e-9e1a-0cc93216dd83","mechanism_event_label":"A chylomicron assembly defect reduced one major vitamin E export route.","subject":{"id":"11265d49-4c79-53a2-8a80-7d62d94be73a","slug":"mouse-mttp-loss","display_name":"Mouse Mttp loss","entity_type_key":"protein_state"},"object":{"id":"4d94ddf8-b1e3-5014-b102-5eb5fb793d5c","slug":"chylomicron-alpha-tocopherol-secretion","display_name":"Chylomicron-associated alpha-tocopherol secretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a23246c7-1b4b-5c3e-9e1a-0cc93216dd83","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-mttp-chylomicron-loss-event","event_type":"biochemical_relationship","label":"A chylomicron assembly defect reduced one major vitamin E export route.","description":"Primary enterocytes from Mttp-deficient mice secreted less alpha-tocopherol with chylomicrons.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"11265d49-4c79-53a2-8a80-7d62d94be73a","slug":"mouse-mttp-loss","display_name":"Mouse Mttp loss","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4d94ddf8-b1e3-5014-b102-5eb5fb793d5c","slug":"chylomicron-alpha-tocopherol-secretion","display_name":"Chylomicron-associated alpha-tocopherol secretion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"555c2700-2b35-56dd-b027-2d85f909655f","slug":"mouse-mttp-gene","display_name":"Mouse Mttp gene","entity_type_key":"gene"},"role":"perturbed_gene","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"role":"cargo","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary Mttp-deficient mouse enterocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Mttp deficiency; radiolabeled alpha-tocopherol; exact genotype induction and incubation details unavailable in abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Genetic machinery impairment, not low dietary vitamin E; HDL secretion is a distinct route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A chylomicron assembly defect reduced one major vitamin E export route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[anwar2007] Mechanisms involved in vitamin E transport by primary enterocytes and in vivo absorption. (2007). https://pubmed.ncbi.nlm.nih.gov/17582142/ DOI: 10.1194/jlr.m700207-jlr200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Small-intestinal enterocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"453ba849-0054-5ad5-ac66-28e1e3129770","evidence_kind":"source_excerpt","locator":"Lines 246-257","start_line":246,"end_line":257,"excerpt":"### ve-transport-mttp-chylomicron-loss\nPrimary enterocytes from Mttp-deficient mice secreted less alpha-tocopherol with chylomicrons.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A chylomicron assembly defect reduced one major vitamin E export route.\norganism: Mus musculus\ntissue_or_cell_type: Small-intestinal enterocytes\nexperimental_model: Primary Mttp-deficient mouse enterocytes\nlimitations: Genetic machinery impairment, not low dietary vitamin E; HDL secretion is a distinct route.\nexposure: Mttp deficiency; radiolabeled alpha-tocopherol; exact genotype induction and incubation details unavailable in abstract.\ncross_nutrient: false\n[anwar2007] Mechanisms involved in vitamin E transport by primary enterocytes and in vivo absorption. 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