{"id":"a27f445f-0fa6-550d-9e5a-3009490e5f31","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-aanat-acetyl-coa","predicate":"provides_acetyl_group_for","statement":"The human AANAT assay used acetyl-CoA as the acetyl donor in N-acetylserotonin formation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9b9f3ed8-893a-5cef-a837-d7dfc841dcc7","mechanism_event_label":"This connects melatonin synthesis to the B5-derived coenzyme-A system.","subject":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"object":{"id":"61505f34-40e1-5795-8184-c3c826ca8268","slug":"aanat","display_name":"Human arylalkylamine N-acetyltransferase / AANAT","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"9b9f3ed8-893a-5cef-a837-d7dfc841dcc7","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-aanat-acetyl-coa-event","event_type":"biochemical_relationship","label":"This connects melatonin synthesis to the B5-derived coenzyme-A system.","description":"The human AANAT assay used acetyl-CoA as the acetyl donor in N-acetylserotonin formation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fef9ecc3-7c04-553c-a207-d91e38a2665a","slug":"serotonin","display_name":"Serotonin","entity_type_key":"small_molecule"},"role":"acceptor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"50dd19e0-6457-59d5-9c01-3a156c28ee7c","slug":"n-acetylserotonin","display_name":"N-Acetylserotonin","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"61505f34-40e1-5795-8184-c3c826ca8268","slug":"aanat","display_name":"Human arylalkylamine N-acetyltransferase / AANAT","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/melatonin-research/10722724.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7fb8418b17e92c5b3fed5d9c08afdb32f28c922dfe94274d3bb02b7e12b2b8c8\", \"start_char\": 0, \"end_char\": 1857, \"text_sha256\": \"7fb8418b17e92c5b3fed5d9c08afdb32f28c922dfe94274d3bb02b7e12b2b8c8\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant enzyme substrate-specificity study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Serotonin and radiolabeled acetyl-CoA; product HPLC","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Biochemical mechanism, not evidence that dietary B5 or acetyl-CoA availability limits melatonin production in all humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Melatonin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human AANAT expressed in bacteria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This connects melatonin synthesis to the B5-derived coenzyme-A system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[melatonin-p10722724] Substrate specificity and inhibition studies of human serotonin N-acetyltransferase. (2000). https://pubmed.ncbi.nlm.nih.gov/10722724/ DOI: 10.1074/jbc.275.12.8794","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Serotonin acetylation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"16b657f7-44bd-5f5b-a3a2-f56e90eb7648","evidence_kind":"source_excerpt","locator":"Lines 188-199","start_line":188,"end_line":199,"excerpt":"### melatonin-aanat-acetyl-coa\nThe human AANAT assay used acetyl-CoA as the acetyl donor in N-acetylserotonin formation.\nCondition category: normal\nnutrient_topic: Melatonin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This connects melatonin synthesis to the B5-derived coenzyme-A system.\norganism: Human AANAT expressed in bacteria\ntissue_or_cell_type: Serotonin acetylation\nexperimental_model: Purified recombinant enzyme substrate-specificity study\nlimitations: Biochemical mechanism, not evidence that dietary B5 or acetyl-CoA availability limits melatonin production in all humans.\nexposure: Serotonin and radiolabeled acetyl-CoA; product HPLC\nevidence_span: {\"source_cache\": \"artifacts/melatonin-research/10722724.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7fb8418b17e92c5b3fed5d9c08afdb32f28c922dfe94274d3bb02b7e12b2b8c8\", \"start_char\": 0, \"end_char\": 1857, \"text_sha256\": \"7fb8418b17e92c5b3fed5d9c08afdb32f28c922dfe94274d3bb02b7e12b2b8c8\"}\n[melatonin-p10722724] Substrate specificity and inhibition studies of human serotonin N-acetyltransferase. 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