{"id":"a27352e5-7f98-5f25-b3a0-de6ac573cc48","stable_key":"c94a9824-35fb-523c-b421-dc7341218dcb:indicaxanthin-endothelial-nfkb","predicate":"reduces","statement":"Indicaxanthin pretreatment suppressed oxidized-LDL-induced NF-kappaB transcriptional activity in HUVECs.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"fa3235a9-8f3e-52af-a219-3c2166c9cee4","mechanism_event_label":"Indicaxanthin pretreatment suppressed oxidized-LDL-induced NF-kappaB transcriptional activity in HUVECs.","subject":{"id":"f2a6e469-05f0-5596-9054-da926389f584","slug":"indicaxanthin","display_name":"Indicaxanthin","entity_type_key":"small_molecule"},"object":{"id":"13ccae5e-9060-5df9-8087-5b90d8294571","slug":"human-huvec-nfkb-transcription","display_name":"NF-kappaB transcriptional activity in human HUVECs","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fa3235a9-8f3e-52af-a219-3c2166c9cee4","stable_key":"c94a9824-35fb-523c-b421-dc7341218dcb:indicaxanthin-endothelial-nfkb-event","event_type":"observed_relationship","label":"Indicaxanthin pretreatment suppressed oxidized-LDL-induced NF-kappaB transcriptional activity in HUVECs.","description":"Indicaxanthin pretreatment suppressed oxidized-LDL-induced NF-kappaB transcriptional activity in HUVECs.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f2a6e469-05f0-5596-9054-da926389f584","slug":"indicaxanthin","display_name":"Indicaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"13ccae5e-9060-5df9-8087-5b90d8294571","slug":"human-huvec-nfkb-transcription","display_name":"NF-kappaB transcriptional activity in human HUVECs","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f2f4e414-62e7-51fa-bbf3-81b81a53701e","slug":"human-oxidized-ldl-preparation","display_name":"Oxidized human LDL preparation","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f133114f-4a46-5a4e-8395-d2848353ee13","slug":"nf-kappa-b-family","display_name":"NF-kappaB family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Indicaxanthin 5-20 micromolar; human oxidized LDL 100 micrograms/mL","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"1 h pretreatment followed by 16 h oxidized-LDL exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary open full text, relevant results/methods and PubMed metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human umbilical vein endothelial cells / HUVECs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression rescue does not demonstrate plaque regression or ABCA1 cholesterol-efflux flux. Primary-source metadata check found no correction/retraction link; broader integrity not assessed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Dedicated indicaxanthin chapter; original betalain family identity and shared claims preserved.","comparator":null,"unit":null,"notes":"","entity":{"slug":"indicaxanthin","display_name":"Indicaxanthin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human umbilical vein endothelial cells / HUVECs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Indicaxanthin pretreatment suppressed oxidized-LDL-induced NF-kappaB transcriptional activity in HUVECs.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Indicaxanthin from Opuntia ficus indica (L. Mill) Inhibits Oxidized LDL-Mediated Human Endothelial Cell Dysfunction through Inhibition of NF-κB Activation. (2019). https://pubmed.ncbi.nlm.nih.gov/30911345/ DOI: 10.1155/2019/3457846","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro pretreatment and challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Endothelial culture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d4ebd2f3-6eca-5bd7-ae33-a0980be35877","evidence_kind":"source_excerpt","locator":"Lines 338-347","start_line":338,"end_line":347,"excerpt":"## indicaxanthin-endothelial-nfkb\nIndicaxanthin pretreatment suppressed oxidized-LDL-induced NF-kappaB transcriptional activity in HUVECs.\nModel/species: Human umbilical vein endothelial cells / HUVECs\nTissue: Endothelial culture\nExposure: Indicaxanthin 5-20 micromolar; human oxidized LDL 100 micrograms/mL\nRoute: In vitro pretreatment and challenge\nDuration: 1 h pretreatment followed by 16 h oxidized-LDL exposure\nLimits: Expression rescue does not demonstrate plaque regression or ABCA1 cholesterol-efflux flux. Primary-source metadata check found no correction/retraction link; broader integrity not assessed.\nPrimary reference: Indicaxanthin from Opuntia ficus indica (L. Mill) Inhibits Oxidized LDL-Mediated Human Endothelial Cell Dysfunction through Inhibition of NF-κB Activation. (2019). https://pubmed.ncbi.nlm.nih.gov/30911345/ DOI: 10.1155/2019/3457846\nAccess: Primary open full text, relevant results/methods and PubMed metadata.","model_system":"Human umbilical vein endothelial cells / HUVECs","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact archived curation span; original paraphrase with primary citation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"18b7303c-98b7-54cb-ae8e-a8c6257657b1","stable_key":"import-c94a9824-35fb-523c-b421-dc7341218dcb","title":"Indicaxanthin: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of thirteen additional primary studies, with twenty-six existing claims from eight studies linked unchanged. Study-specific citations and limitations retained. 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