{"id":"a26c7a90-df4e-5b64-b4ef-11fd7b918e8e","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-folate-low","predicate":"modulates","statement":"Folate depletion increased lymphoma-cell sensitivity to EGCG; the reported growth IC50 was 20 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c360ca8f-c8a3-5af0-8ae6-4050351d5a7f","mechanism_event_label":"Low folate made the cultured cells more sensitive.","subject":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"object":{"id":"d947ab3a-b52a-58ce-8626-86538ed56e76","slug":"egcg-lymphoma-proliferation","display_name":"Proliferation of EGCG-exposed lymphoma cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c360ca8f-c8a3-5af0-8ae6-4050351d5a7f","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-folate-low-event","event_type":"observed_relationship","label":"Low folate made the cultured cells more sensitive.","description":"Folate depletion increased lymphoma-cell sensitivity to EGCG; the reported growth IC50 was 20 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d947ab3a-b52a-58ce-8626-86538ed56e76","slug":"egcg-lymphoma-proliferation","display_name":"Proliferation of EGCG-exposed lymphoma cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Purified bovine/chicken DHFR and lymphoma-cell experiments; human DHFR interaction was modeled computationally.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not relabel the purified enzymes as human DHFR or infer that normal tea intake causes folate deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Low folate made the cultured cells more sensitive.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The antifolate activity of tea catechins. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15781612/ · DOI 10.1158/0008-5472.can-04-3469","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"d98dccec-6120-544c-8c7f-38114437f407","evidence_kind":"source_excerpt","locator":"Lines 140-146","start_line":140,"end_line":146,"excerpt":"## egcg-folate-low\nLow folate made the cultured cells more sensitive.\nFolate depletion increased lymphoma-cell sensitivity to EGCG; the reported growth IC50 was 20 micromolar.\nModel: Purified bovine/chicken DHFR and lymphoma-cell experiments; human DHFR interaction was modeled computationally.\nLimitations: Do not relabel the purified enzymes as human DHFR or infer that normal tea intake causes folate deficiency.\nEvidence access: primary abstract.\nThe antifolate activity of tea catechins. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15781612/ · DOI 10.1158/0008-5472.can-04-3469","model_system":"Purified bovine/chicken DHFR and lymphoma-cell experiments; human DHFR interaction was modeled computationally.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b101c8d4d96ee03ff97eb1922c7bb0f96943295af473fdc3ef98b0dbd62fe8c7","revision_id":"e19bc5b2-ee3c-5f67-b5c5-9dc3b7d5f850","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}