{"id":"a267f759-dc72-55a4-a770-b0616c1f4a44","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-mtacp-lipoylation","predicate":"depletion_reduces","statement":"Mitochondrial ACP knockdown in HEK293T cells reduced the proportion of mitochondrial proteins recognized as lipoylated before later respiratory defects.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2f5b2d50-80b9-529f-b041-3dd9f21a57e2","mechanism_event_label":"Loss of the mitochondrial carrier impaired attachment of the lipoate cofactor to proteins.","subject":{"id":"2c0bfd00-1cde-5542-82e6-527d9b72908c","slug":"ndufab1","display_name":"Human mitochondrial acyl carrier protein / NDUFAB1","entity_type_key":"protein"},"object":{"id":"a545cda4-692b-5513-a8f3-71c0afb0736c","slug":"mitochondrial-protein-lipoylation","display_name":"Mitochondrial protein lipoylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2f5b2d50-80b9-529f-b041-3dd9f21a57e2","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-mtacp-lipoylation-event","event_type":"biochemical_relationship","label":"Loss of the mitochondrial carrier impaired attachment of the lipoate cofactor to proteins.","description":"Mitochondrial ACP knockdown in HEK293T cells reduced the proportion of mitochondrial proteins recognized as lipoylated before later respiratory defects.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"related_cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fd2197c3-5a63-55f4-8bb1-bfc1e91a0e1e","slug":"protein-bound-lipoamide","display_name":"Protein-bound oxidized lipoyl-lysine","entity_type_key":"chemical_species"},"role":"measured_protein_bound_cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2c0bfd00-1cde-5542-82e6-527d9b72908c","slug":"ndufab1","display_name":"Human mitochondrial acyl carrier protein / NDUFAB1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a545cda4-692b-5513-a8f3-71c0afb0736c","slug":"mitochondrial-protein-lipoylation","display_name":"Mitochondrial protein lipoylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Abstract, earliest phenotypic changes; Fig. 2 caption","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mitochondrial ACP siRNA in HEK293T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ACP mRNA and protein reduced by more than 85% within 24 hours; later measurements followed knockdown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Antibody-based lipoylation readout; not a measurement of dietary lipoic acid or pantothenate shortage. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Loss of the mitochondrial carrier impaired attachment of the lipoate cofactor to proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-acp2009] Down-regulation of mitochondrial acyl carrier protein in mammalian cells compromises protein lipoylation and respiratory complex I and results in cell death. (2009). https://pubmed.ncbi.nlm.nih.gov/19221180/ DOI: 10.1074/jbc.m806991200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293T mitochondrial proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"6b0782b3-767f-59a8-b46d-08864d98234d","evidence_kind":"source_excerpt","locator":"Lines 823-835","start_line":823,"end_line":835,"excerpt":"### b5-met-mtacp-lipoylation\nMitochondrial ACP knockdown in HEK293T cells reduced the proportion of mitochondrial proteins recognized as lipoylated before later respiratory defects.\nCondition category: machinery_impairment\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Loss of the mitochondrial carrier impaired attachment of the lipoate cofactor to proteins.\norganism: Homo sapiens\ntissue_or_cell_type: HEK293T mitochondrial proteins\nexperimental_model: Mitochondrial ACP siRNA in HEK293T cells\nlimitations: Antibody-based lipoylation readout; not a measurement of dietary lipoic acid or pantothenate shortage. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: ACP mRNA and protein reduced by more than 85% within 24 hours; later measurements followed knockdown.\ncross_nutrient: true\nevidence_location: Abstract, earliest phenotypic changes; Fig. 2 caption\n[b5-met-acp2009] Down-regulation of mitochondrial acyl carrier protein in mammalian cells compromises protein lipoylation and respiratory complex I and results in cell death. (2009). https://pubmed.ncbi.nlm.nih.gov/19221180/ DOI: 10.1074/jbc.m806991200","model_system":"Mitochondrial ACP siRNA in HEK293T cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-acp2009] Down-regulation of mitochondrial acyl carrier protein in mammalian cells compromises protein lipoylation and respiratory complex I and results in cell death. (2009). https://pubmed.ncbi.nlm.nih.gov/19221180/ DOI: 10.1074/jbc.m806991200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}