{"id":"a24e3f27-6fa6-57e8-ad4b-50014bbc356c","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-stc-sodium","predicate":"undergoes_sodium_dependent_uptake_in","statement":"Sodium-dependent phenylalanine uptake contributed to depolarization and GLP-1 secretion in STC-1 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ba5cf471-2df4-5a46-a616-7c244c571ec5","mechanism_event_label":"Amino-acid transport can supply an electrical signal as well as a nutrient.","subject":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"object":{"id":"2b4eb678-dd92-532d-955d-a94b4216300e","slug":"stc1-phe-depolarization","display_name":"STC-1 phenylalanine-evoked membrane depolarization","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ba5cf471-2df4-5a46-a616-7c244c571ec5","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-stc-sodium-event","event_type":"observed_relationship","label":"Amino-acid transport can supply an electrical signal as well as a nutrient.","description":"Sodium-dependent phenylalanine uptake contributed to depolarization and GLP-1 secretion in STC-1 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b4eb678-dd92-532d-955d-a94b4216300e","slug":"stc1-phe-depolarization","display_name":"STC-1 phenylalanine-evoked membrane depolarization","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4853afc6-9eaa-547f-91d7-c4dd90082351","slug":"glp-1","display_name":"Glucagon-like peptide 1 / GLP-1","entity_type_key":"peptide"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse STC-1 cell transport, membrane-potential and secretion assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The accessed abstract does not identify a specific transporter isoform; B0AT1 is not assigned by inference.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Amino-acid transport can supply an electrical signal as well as a nutrient.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34953208/ · DOI 10.1016/j.bbrc.2021.12.043","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ee2c5c48-ee42-5a21-9df5-61f85f510070","evidence_kind":"source_excerpt","locator":"Lines 318-324","start_line":318,"end_line":324,"excerpt":"## l-phenylalanine-stc-sodium\nAmino-acid transport can supply an electrical signal as well as a nutrient.\nSodium-dependent phenylalanine uptake contributed to depolarization and GLP-1 secretion in STC-1 cells.\nModel: Mouse STC-1 cell transport, membrane-potential and secretion assays.\nLimitations: The accessed abstract does not identify a specific transporter isoform; B0AT1 is not assigned by inference.\nEvidence access: Primary abstract\nIdentification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34953208/ · DOI 10.1016/j.bbrc.2021.12.043","model_system":"Mouse STC-1 cell transport, membrane-potential and secretion assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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