{"id":"a1fa994e-113e-546a-bd88-84150fcdc510","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-chdh-betaine","predicate":"reduces","statement":"Chdh deletion lowered testicular betaine while choline and phosphocholine increased.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9e64909b-f8cb-519a-b477-6cf5a31c5d9f","mechanism_event_label":"Choline accumulated upstream while a downstream product fell.","subject":{"id":"78c8e1df-b5f8-5bd0-8d35-503d3e89a175","slug":"mouse-chdh-null","display_name":"Experimental mouse Chdh-null genotype","entity_type_key":"protein_state"},"object":{"id":"f65c03ca-b207-5d2e-9110-c487ebbb44c9","slug":"mouse-testicular-betaine","display_name":"Mouse testicular betaine concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9e64909b-f8cb-519a-b477-6cf5a31c5d9f","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-chdh-betaine-event","event_type":"biochemical_relationship","label":"Choline accumulated upstream while a downstream product fell.","description":"Chdh deletion lowered testicular betaine while choline and phosphocholine increased.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bb913152-b288-5398-9c04-2345f3a4202b","slug":"mouse-chdh","display_name":"Mouse choline dehydrogenase / Chdh","entity_type_key":"protein"},"role":"missing_enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"78c8e1df-b5f8-5bd0-8d35-503d3e89a175","slug":"mouse-chdh-null","display_name":"Experimental mouse Chdh-null genotype","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f65c03ca-b207-5d2e-9110-c487ebbb44c9","slug":"mouse-testicular-betaine","display_name":"Mouse testicular betaine concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/20371614.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dce0c14d2eb09194040087e691053960d9e4f477a49099cdd9482ad9ab38dc48\", \"start_char\": 0, \"end_char\": 1334, \"text_sha256\": \"dce0c14d2eb09194040087e691053960d9e4f477a49099cdd9482ad9ab38dc48\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chdh-null mouse genetics and sperm biochemical assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Chdh deletion versus wild type","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Loss of an enzyme is not the same exposure as low dietary choline; mouse fertility results are not a human diagnostic rule. The 2018 correction replaces the original knockout exon diagram: exons 1–6 and much of exon 7 were deleted, not only exons 1–3 (PMID 29698116; doi:10.1096/fj.09-153718ERR).","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Choline accumulated upstream while a downstream product fell.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p20371614] Deletion of murine choline dehydrogenase results in diminished sperm motility. 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The 2018 correction replaces the original knockout exon diagram: exons 1–6 and much of exon 7 were deleted, not only exons 1–3 (PMID 29698116; doi:10.1096/fj.09-153718ERR).\nexposure: Chdh deletion versus wild type\nevidence_span: {\"source_cache\": \"artifacts/choline-research/20371614.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dce0c14d2eb09194040087e691053960d9e4f477a49099cdd9482ad9ab38dc48\", \"start_char\": 0, \"end_char\": 1334, \"text_sha256\": \"dce0c14d2eb09194040087e691053960d9e4f477a49099cdd9482ad9ab38dc48\"}\n[choline-p20371614] Deletion of murine choline dehydrogenase results in diminished sperm motility. 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