{"id":"a1f9d2cc-749a-5579-b8da-58729859a1ca","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-tat-carbon","predicate":"produces","statement":"Recombinant human TAT catalyzed reversible tyrosine transamination, with the highest activity for the tyrosine/2-oxoglutarate substrate pair.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"316f4ca7-cf5a-51e2-9520-32c386e82c10","mechanism_event_label":"Tyrosine breakdown begins by moving its amino group.","subject":{"id":"f4bcff80-2f4b-5295-a5fa-14e2d6720ec9","slug":"tat","display_name":"Human tyrosine aminotransferase / TAT","entity_type_key":"protein"},"object":{"id":"9e57c89c-2e87-5bc7-b2b9-29b122942fdd","slug":"4-hydroxyphenylpyruvate","display_name":"4-Hydroxyphenylpyruvate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"316f4ca7-cf5a-51e2-9520-32c386e82c10","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-tat-carbon-event","event_type":"observed_relationship","label":"Tyrosine breakdown begins by moving its amino group.","description":"Recombinant human TAT catalyzed reversible tyrosine transamination, with the highest activity for the tyrosine/2-oxoglutarate substrate pair.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f4bcff80-2f4b-5295-a5fa-14e2d6720ec9","slug":"tat","display_name":"Human tyrosine aminotransferase / TAT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9e57c89c-2e87-5bc7-b2b9-29b122942fdd","slug":"4-hydroxyphenylpyruvate","display_name":"4-Hydroxyphenylpyruvate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified full-length and truncated human enzyme expressed in E. coli.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A reversible enzyme assay is not a measurement of net in vivo flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Tyrosine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Tyrosine breakdown begins by moving its amino group.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The narrow substrate specificity of human tyrosine aminotransferase--the enzyme deficient in tyrosinemia type II. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16640556/ · DOI 10.1111/j.1742-4658.2006.05202.x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"98963ada-1d65-5530-a743-d3ae5f6d4bbf","evidence_kind":"source_excerpt","locator":"Lines 204-210","start_line":204,"end_line":210,"excerpt":"## l-tyrosine-tat-carbon\nTyrosine breakdown begins by moving its amino group.\nRecombinant human TAT catalyzed reversible tyrosine transamination, with the highest activity for the tyrosine/2-oxoglutarate substrate pair.\nModel: Purified full-length and truncated human enzyme expressed in E. coli.\nLimitations: A reversible enzyme assay is not a measurement of net in vivo flux.\nEvidence access: Primary abstract\nThe narrow substrate specificity of human tyrosine aminotransferase--the enzyme deficient in tyrosinemia type II. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16640556/ · DOI 10.1111/j.1742-4658.2006.05202.x","model_system":"Purified full-length and truncated human enzyme expressed in E. coli.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"12917df2-c6e0-5b61-850f-dbff6d4b4d30","stable_key":"import-63ce713e-6aea-59f6-9896-ca30e010b2ce","title":"L-Tyrosine: catecholamines, thyroid chemistry, pigment, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7777f4440a8bfb14a04ff73e73392ccc45d8ee89f7853ecbcc0027d6f563b8a0","revision_id":"e1206111-3a75-5809-b8e9-231ff809ff1e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}