{"id":"a19d1808-ed3a-5690-b6cd-0a3b55ba901d","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-shmt2-mutation","predicate":"mutation_reduces","statement":"Shmt2-mutant Chinese hamster ovary cells showed reduced mitochondrial tRNA taurine modification compared with wild type.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"15028491-791e-5851-aea4-356806f14ae3","mechanism_event_label":"One-carbon supply can affect the same RNA mark even when the taurine molecule is present.","subject":{"id":"70c8a904-a7dd-5426-832a-748db0288a5c","slug":"hamster-shmt2","display_name":"Chinese hamster mitochondrial serine hydroxymethyltransferase / Shmt2","entity_type_key":"protein"},"object":{"id":"dd968147-5f93-56d5-bc83-7fa01f9b0e42","slug":"mt-trna-taurinomethyluridine","display_name":"Mitochondrial tRNA 5-taurinomethyluridine / tau-m5U34","entity_type_key":"rna_modification"},"evidence_count":1,"mechanism_event":{"id":"15028491-791e-5851-aea4-356806f14ae3","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-shmt2-mutation-event","event_type":"observed_relationship","label":"One-carbon supply can affect the same RNA mark even when the taurine molecule is present.","description":"Shmt2-mutant Chinese hamster ovary cells showed reduced mitochondrial tRNA taurine modification compared with wild type.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"70c8a904-a7dd-5426-832a-748db0288a5c","slug":"hamster-shmt2","display_name":"Chinese hamster mitochondrial serine hydroxymethyltransferase / Shmt2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dd968147-5f93-56d5-bc83-7fa01f9b0e42","slug":"mt-trna-taurinomethyluridine","display_name":"Mitochondrial tRNA 5-taurinomethyluridine / tau-m5U34","entity_type_key":"rna_modification"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0413b195-325e-5757-a72b-f445d65c17bc","slug":"5-10-methylenetetrahydrofolate","display_name":"5,10-Methylenetetrahydrofolate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text, Figure 1F","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"CHO mutant-cell mass spectrometry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This species-specific machinery defect is not a B6 or folate deprivation experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"One-carbon supply can affect the same RNA mark even when the taurine molecule is present.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29390138/ · DOI 10.1093/nar/gky068","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"51de91b6-f7be-53ed-ab8b-93d4e29ec0fb","evidence_kind":"source_excerpt","locator":"Lines 217-223","start_line":217,"end_line":223,"excerpt":"## taurine-shmt2-mutation\nOne-carbon supply can affect the same RNA mark even when the taurine molecule is present.\nShmt2-mutant Chinese hamster ovary cells showed reduced mitochondrial tRNA taurine modification compared with wild type.\nModel: CHO mutant-cell mass spectrometry.\nLimitations: This species-specific machinery defect is not a B6 or folate deprivation experiment.\nEvidence access: Primary full text, Figure 1F\nMetabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29390138/ · DOI 10.1093/nar/gky068","model_system":"CHO mutant-cell mass spectrometry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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