{"id":"a147b32d-5b7e-55a1-8333-53888e906a22","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-6-l6-ampk","predicate":"increases_phosphorylation","statement":"Adding (S)-6-gingerol increased AMPK alpha Thr172 phosphorylation in rat L6 cells, with CaMKK-associated signaling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"869e55ad-e3c8-5216-8abc-d02ae87eff66","mechanism_event_label":"Adding (S)-6-gingerol increased AMPK alpha Thr172 phosphorylation in rat L6 cells, with CaMKK-associated signaling.","subject":{"id":"837fdd65-c1eb-52e0-a724-42a1e9065c82","slug":"6-gingerol","display_name":"6-Gingerol","entity_type_key":"small_molecule"},"object":{"id":"378e97ad-f6dc-5b66-a889-e95543049489","slug":"rat-ampk-complexes","display_name":"Rat AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"869e55ad-e3c8-5216-8abc-d02ae87eff66","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-6-l6-ampk-event","event_type":"observed_relationship","label":"Adding (S)-6-gingerol increased AMPK alpha Thr172 phosphorylation in rat L6 cells, with CaMKK-associated signaling.","description":"Adding (S)-6-gingerol increased AMPK alpha Thr172 phosphorylation in rat L6 cells, with CaMKK-associated signaling.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"837fdd65-c1eb-52e0-a724-42a1e9065c82","slug":"6-gingerol","display_name":"6-Gingerol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"378e97ad-f6dc-5b66-a889-e95543049489","slug":"rat-ampk-complexes","display_name":"Rat AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7f38b9e3-0c79-5cf1-9cb9-7c86e4455140","slug":"rat-camkk-family","display_name":"Rat calcium/calmodulin-dependent protein kinase kinase family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"(S)-6-Gingerol; dose-response, exact range not in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Time-dependent response; exact times not in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation, not universally established human effects","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat L6 skeletal muscle myotubes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Calcium/CaMKK-associated signaling does not demonstrate dietary calcium depletion or calcium-supplement synergy; no human glycemic outcome was tested. The accessed abstract does not identify a CaMKK isoform or establish genetic necessity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gingerols collection; each molecular form and experimental preparation remains explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gingerols","display_name":"Gingerols","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rat L6 skeletal muscle myotubes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Adding (S)-6-gingerol increased AMPK alpha Thr172 phosphorylation in rat L6 cells, with CaMKK-associated signaling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"(S)-[6]-Gingerol enhances glucose uptake in L6 myotubes by activation of AMPK in response to [Ca2+]i. (2013). https://pubmed.ncbi.nlm.nih.gov/23958199/ DOI: 10.18433/j34g7p","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro addition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Skeletal muscle cell model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7cef0ff5-db4f-5594-91a0-def8606ce35e","evidence_kind":"source_excerpt","locator":"Lines 162-171","start_line":162,"end_line":171,"excerpt":"## gingerols-6-l6-ampk\nAdding (S)-6-gingerol increased AMPK alpha Thr172 phosphorylation in rat L6 cells, with CaMKK-associated signaling.\nModel/species: Rat L6 skeletal muscle myotubes\nTissue: Skeletal muscle cell model\nExposure: (S)-6-Gingerol; dose-response, exact range not in accessed abstract\nRoute: In vitro addition\nDuration: Time-dependent response; exact times not in accessed abstract\nLimits: Calcium/CaMKK-associated signaling does not demonstrate dietary calcium depletion or calcium-supplement synergy; no human glycemic outcome was tested. The accessed abstract does not identify a CaMKK isoform or establish genetic necessity.\nPrimary reference: (S)-[6]-Gingerol enhances glucose uptake in L6 myotubes by activation of AMPK in response to [Ca2+]i. (2013). https://pubmed.ncbi.nlm.nih.gov/23958199/ DOI: 10.18433/j34g7p\nAccess: Primary PubMed abstract.","model_system":"Rat L6 skeletal muscle myotubes","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact archived synthesis span with its primary citation; original paraphrase, not a claimed publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1107d60b-8e2d-55d7-8f5d-5612c49aa7dc","stable_key":"import-fd7915ff-856d-56c2-a118-4989693a7644","title":"Gingerols: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of thirteen primary research papers; study-specific PubMed/DOI links and limitations retained. Not publisher full text.","file_path":"","sha256":"96d1b7b04445eecd0eac1738e0c80cb6308509ecdfc3b5fc6be67ae2218cbb2b","revision_id":"de6e70ff-0e52-5c26-bd3a-54fca2085a86","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}