{"id":"a1475af5-8522-5f72-bbf7-4158740a0299","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-keap1-ligase","predicate":"assembles_with","statement":"KEAP1 acted as a substrate adaptor in a functional CUL3-RBX1 ubiquitin-ligase complex.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e7975864-e46c-5dae-937b-fbbb7a8a7c39","mechanism_event_label":"The sensor helps bring the protein targeted for disposal to the disposal machinery.","subject":{"id":"c5b67b38-fe93-5e8f-91af-d77d3f590d98","slug":"keap1","display_name":"Human KEAP1","entity_type_key":"protein"},"object":{"id":"cc837ba5-60c3-5fb0-8b58-bf5a1ffdd9c1","slug":"keap1-cul3-rbx1","display_name":"Human KEAP1-CUL3-RBX1 ubiquitin-ligase complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"e7975864-e46c-5dae-937b-fbbb7a8a7c39","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-keap1-ligase-event","event_type":"biochemical_relationship","label":"The sensor helps bring the protein targeted for disposal to the disposal machinery.","description":"KEAP1 acted as a substrate adaptor in a functional CUL3-RBX1 ubiquitin-ligase complex.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c5b67b38-fe93-5e8f-91af-d77d3f590d98","slug":"keap1","display_name":"Human KEAP1","entity_type_key":"protein"},"role":"sensor_adaptor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3bf390ea-a550-5965-9e1e-ae36ce4cbc9a","slug":"cul3","display_name":"Human cullin 3 / CUL3","entity_type_key":"protein"},"role":"scaffold","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"515aa81c-e027-507a-90c9-8799fa46626c","slug":"rbx1","display_name":"Human RING-box protein 1 / RBX1","entity_type_key":"protein"},"role":"ligase_component","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"regulated_substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"cc837ba5-60c3-5fb0-8b58-bf5a1ffdd9c1","slug":"keap1-cul3-rbx1","display_name":"Human KEAP1-CUL3-RBX1 ubiquitin-ligase complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/15572695.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d120b0ea48a77a99b12e9147e4acb02fd63026ed729a37d5e2edf174c5674765\", \"start_char\": 0, \"end_char\": 1484, \"text_sha256\": \"d120b0ea48a77a99b12e9147e4acb02fd63026ed729a37d5e2edf174c5674765\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell transfection, coimmunoprecipitation and ubiquitination assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sulforaphane or quinone stress; C151S KEAP1 comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The measured interaction is ubiquitin-ligase regulation; do not describe it as obligatory physical release of all bound Nrf2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human protein constructs in mammalian cell systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The sensor helps bring the protein targeted for disposal to the disposal machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p15572695] Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. (2004). https://pubmed.ncbi.nlm.nih.gov/15572695/ DOI: 10.1128/mcb.24.24.10941-10953.2004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"KEAP1-CUL3-RBX1 control of Nrf2","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0d226ed9-9b6c-5bf3-b21f-46f76d18d5d1","evidence_kind":"source_excerpt","locator":"Lines 450-461","start_line":450,"end_line":461,"excerpt":"### sulforaphane-keap1-ligase\nKEAP1 acted as a substrate adaptor in a functional CUL3-RBX1 ubiquitin-ligase complex.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The sensor helps bring the protein targeted for disposal to the disposal machinery.\norganism: Human protein constructs in mammalian cell systems\ntissue_or_cell_type: KEAP1-CUL3-RBX1 control of Nrf2\nexperimental_model: Cell transfection, coimmunoprecipitation and ubiquitination assays\nlimitations: The measured interaction is ubiquitin-ligase regulation; do not describe it as obligatory physical release of all bound Nrf2.\nexposure: Sulforaphane or quinone stress; C151S KEAP1 comparison\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/15572695.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d120b0ea48a77a99b12e9147e4acb02fd63026ed729a37d5e2edf174c5674765\", \"start_char\": 0, \"end_char\": 1484, \"text_sha256\": \"d120b0ea48a77a99b12e9147e4acb02fd63026ed729a37d5e2edf174c5674765\"}\n[sulforaphane-p15572695] Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. (2004). https://pubmed.ncbi.nlm.nih.gov/15572695/ DOI: 10.1128/mcb.24.24.10941-10953.2004","model_system":"Cell transfection, coimmunoprecipitation and ubiquitination assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sulforaphane-p15572695] Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. (2004). https://pubmed.ncbi.nlm.nih.gov/15572695/ DOI: 10.1128/mcb.24.24.10941-10953.2004","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"a4bafbf7-f117-53c9-a6d7-8fb25e4aca72","stable_key":"import-44eaeae5-557a-552b-8998-884f30462e2a","title":"Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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