{"id":"a11c08da-0e82-594b-aeb9-e1c6020f4508","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-mt1-human-binding","predicate":"binds_melatonin_agonist_at","statement":"Expressed human MT1 showed high-affinity iodinated-melatonin binding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"aa441eb5-df08-535b-9ff6-d4603772e86d","mechanism_event_label":"MT1 has its own molecular identity; it is not interchangeable with MT2.","subject":{"id":"dff2050f-ecec-53a7-8e40-8cc2ad2da882","slug":"mtnr1a","display_name":"Human melatonin receptor 1A / MT1 / MTNR1A","entity_type_key":"protein"},"object":{"id":"f479551c-e944-5a74-9ef1-fb73b2d01eb4","slug":"melatonin-mt1-agonist-binding","display_name":"MT1 high-affinity agonist binding","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"aa441eb5-df08-535b-9ff6-d4603772e86d","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-mt1-human-binding-event","event_type":"biochemical_relationship","label":"MT1 has its own molecular identity; it is not interchangeable with MT2.","description":"Expressed human MT1 showed high-affinity iodinated-melatonin binding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0f0bc1f1-d18d-5652-bc1d-98f61f7ab65c","slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"},"role":"endogenous_ligand_context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dff2050f-ecec-53a7-8e40-8cc2ad2da882","slug":"mtnr1a","display_name":"Human melatonin receptor 1A / MT1 / MTNR1A","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f479551c-e944-5a74-9ef1-fb73b2d01eb4","slug":"melatonin-mt1-agonist-binding","display_name":"MT1 high-affinity agonist binding","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/melatonin-research/7946354.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4eed36768519d1c02ed67434eb3dc08eb561de445d3532113e7eaeb2827e021f\", \"start_char\": 0, \"end_char\": 1054, \"text_sha256\": \"4eed36768519d1c02ed67434eb3dc08eb561de445d3532113e7eaeb2827e021f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Receptor cloning, radioligand binding and functional expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Heterologous COS-7 binding and NIH3T3 functional assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Species differ across experiments. High-affinity radioligand binding is separated from the sheep functional assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Melatonin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human and sheep MT1 in cells; functional cyclase assay used sheep receptor","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"MT1 has its own molecular identity; it is not interchangeable with MT2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[melatonin-p7946354] Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. (1994). https://pubmed.ncbi.nlm.nih.gov/7946354/ DOI: 10.1016/0896-6273(94)90055-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Melatonin receptor identification","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5aaf6909-97e0-571b-8f49-3a82c15b76d4","evidence_kind":"source_excerpt","locator":"Lines 357-368","start_line":357,"end_line":368,"excerpt":"### melatonin-mt1-human-binding\nExpressed human MT1 showed high-affinity iodinated-melatonin binding.\nCondition category: normal\nnutrient_topic: Melatonin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: MT1 has its own molecular identity; it is not interchangeable with MT2.\norganism: Human and sheep MT1 in cells; functional cyclase assay used sheep receptor\ntissue_or_cell_type: Melatonin receptor identification\nexperimental_model: Receptor cloning, radioligand binding and functional expression\nlimitations: Species differ across experiments. High-affinity radioligand binding is separated from the sheep functional assay.\nexposure: Heterologous COS-7 binding and NIH3T3 functional assays\nevidence_span: {\"source_cache\": \"artifacts/melatonin-research/7946354.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4eed36768519d1c02ed67434eb3dc08eb561de445d3532113e7eaeb2827e021f\", \"start_char\": 0, \"end_char\": 1054, \"text_sha256\": \"4eed36768519d1c02ed67434eb3dc08eb561de445d3532113e7eaeb2827e021f\"}\n[melatonin-p7946354] Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. (1994). https://pubmed.ncbi.nlm.nih.gov/7946354/ DOI: 10.1016/0896-6273(94)90055-8","model_system":"Receptor cloning, radioligand binding and functional expression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [melatonin-p7946354] Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. (1994). https://pubmed.ncbi.nlm.nih.gov/7946354/ DOI: 10.1016/0896-6273(94)90055-8","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bab588de-d217-5038-9c8b-b41222eba08b","stable_key":"import-3279ff78-2460-5064-bfc0-3f2987af85a0","title":"Melatonin: synthesis, receptors, circadian timing and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ff4f279d263f95a69da8e7e8a6c6318ab6ba1b55254dbbda1bfa9ce437e1db80","revision_id":"0318eef8-ff38-513e-8ffa-458146ca5e77","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}