{"id":"a0ba8090-6d14-5aa4-9084-5ab205392660","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-singlet-oxygen-afmk","predicate":"oxidized_to","statement":"Oxidation of melatonin by singlet oxygen produced AFMK, verified by chemical and spectroscopic analyses.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c631451b-2ea2-5574-8348-c9d6d3051fba","mechanism_event_label":"An antioxidant reaction changes melatonin into another molecule that is recorded separately.","subject":{"id":"0f0bc1f1-d18d-5652-bc1d-98f61f7ab65c","slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"},"object":{"id":"9ef1da01-cf91-55eb-910e-0bff19f5192e","slug":"afmk","display_name":"N1-Acetyl-N2-formyl-5-methoxykynuramine / AFMK","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"c631451b-2ea2-5574-8348-c9d6d3051fba","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-singlet-oxygen-afmk-event","event_type":"biochemical_relationship","label":"An antioxidant reaction changes melatonin into another molecule that is recorded separately.","description":"Oxidation of melatonin by singlet oxygen produced AFMK, verified by chemical and spectroscopic analyses.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ac4a9e3e-1454-5802-82a3-3b3b18afc417","slug":"singlet-oxygen","display_name":"Singlet molecular oxygen","entity_type_key":"chemical_species"},"role":"oxidant","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0f0bc1f1-d18d-5652-bc1d-98f61f7ab65c","slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9ef1da01-cf91-55eb-910e-0bff19f5192e","slug":"afmk","display_name":"N1-Acetyl-N2-formyl-5-methoxykynuramine / AFMK","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/melatonin-research/12887657.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3d221b752cc6b59d719b21fd8ec2236bd75232ff34789f544a88e37bc270e8a5\", \"start_char\": 0, \"end_char\": 1108, \"text_sha256\": \"3d221b752cc6b59d719b21fd8ec2236bd75232ff34789f544a88e37bc270e8a5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isotope-labeled chemical oxidation and product identification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Photosensitized or chemically generated singlet oxygen","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Chemical scavenging/product formation is not proof that normal blood melatonin provides a clinically meaningful antioxidant flux or replaces vitamin C, E or selenium enzymes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Melatonin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Cell-free chemical systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An antioxidant reaction changes melatonin into another molecule that is recorded separately.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[melatonin-p12887657] Oxidation of melatonin by singlet molecular oxygen (O2(1deltag)) produces N1-acetyl-N2-formyl-5-methoxykynurenine. (2003). https://pubmed.ncbi.nlm.nih.gov/12887657/ DOI: 10.1034/j.1600-079x.2003.00066.x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Melatonin oxidation chemistry","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8074e73a-87b3-5324-9042-af4263faa4cd","evidence_kind":"source_excerpt","locator":"Lines 513-524","start_line":513,"end_line":524,"excerpt":"### melatonin-singlet-oxygen-afmk\nOxidation of melatonin by singlet oxygen produced AFMK, verified by chemical and spectroscopic analyses.\nCondition category: normal\nnutrient_topic: Melatonin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An antioxidant reaction changes melatonin into another molecule that is recorded separately.\norganism: Cell-free chemical systems\ntissue_or_cell_type: Melatonin oxidation chemistry\nexperimental_model: Isotope-labeled chemical oxidation and product identification\nlimitations: Chemical scavenging/product formation is not proof that normal blood melatonin provides a clinically meaningful antioxidant flux or replaces vitamin C, E or selenium enzymes.\nexposure: Photosensitized or chemically generated singlet oxygen\nevidence_span: {\"source_cache\": \"artifacts/melatonin-research/12887657.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3d221b752cc6b59d719b21fd8ec2236bd75232ff34789f544a88e37bc270e8a5\", \"start_char\": 0, \"end_char\": 1108, \"text_sha256\": \"3d221b752cc6b59d719b21fd8ec2236bd75232ff34789f544a88e37bc270e8a5\"}\n[melatonin-p12887657] Oxidation of melatonin by singlet molecular oxygen (O2(1deltag)) produces N1-acetyl-N2-formyl-5-methoxykynurenine. (2003). https://pubmed.ncbi.nlm.nih.gov/12887657/ DOI: 10.1034/j.1600-079x.2003.00066.x","model_system":"Isotope-labeled chemical oxidation and product identification","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [melatonin-p12887657] Oxidation of melatonin by singlet molecular oxygen (O2(1deltag)) produces N1-acetyl-N2-formyl-5-methoxykynurenine. (2003). https://pubmed.ncbi.nlm.nih.gov/12887657/ DOI: 10.1034/j.1600-079x.2003.00066.x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bab588de-d217-5038-9c8b-b41222eba08b","stable_key":"import-3279ff78-2460-5064-bfc0-3f2987af85a0","title":"Melatonin: synthesis, receptors, circadian timing and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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