{"id":"a08a725b-ef7f-5c2d-ae88-11cf917678da","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-zebrafish-loss","predicate":"knockout_reduces","statement":"Zebrafish ETT knockout lowered ergothioneine content by more than 1000-fold.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a8623d0a-be7b-502c-b8e7-26542d2a95ab","mechanism_event_label":"In this animal model, the transporter controls nearly the entire pool.","subject":{"id":"fdf8a38f-1a41-5993-b6fd-2239cba9406b","slug":"zebrafish-slc22a4","display_name":"Zebrafish ergothioneine transporter ETT","entity_type_key":"protein"},"object":{"id":"c26e71f8-4183-5e47-b080-8796da6b5354","slug":"zebrafish-body-ergothioneine","display_name":"Zebrafish whole-body ergothioneine content","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a8623d0a-be7b-502c-b8e7-26542d2a95ab","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-zebrafish-loss-event","event_type":"observed_relationship","label":"In this animal model, the transporter controls nearly the entire pool.","description":"Zebrafish ETT knockout lowered ergothioneine content by more than 1000-fold.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fdf8a38f-1a41-5993-b6fd-2239cba9406b","slug":"zebrafish-slc22a4","display_name":"Zebrafish ergothioneine transporter ETT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c26e71f8-4183-5e47-b080-8796da6b5354","slug":"zebrafish-body-ergothioneine","display_name":"Zebrafish whole-body ergothioneine content","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Retroviral insertion into transporter exon 1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No obvious gross morphology or behavior difference was reported; do not transfer sole-transporter status to humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"In this animal model, the transporter controls nearly the entire pool.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Knockout of the ergothioneine transporter ETT in zebrafish results in increased 8-oxoguanine levels. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25746775/ · DOI 10.1016/j.freeradbiomed.2015.02.026","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"d1477c74-dfd4-5c37-b91d-f9a18d4efa0a","evidence_kind":"source_excerpt","locator":"Lines 96-102","start_line":96,"end_line":102,"excerpt":"## ergothioneine-zebrafish-loss\nIn this animal model, the transporter controls nearly the entire pool.\nZebrafish ETT knockout lowered ergothioneine content by more than 1000-fold.\nModel: Retroviral insertion into transporter exon 1.\nLimitations: No obvious gross morphology or behavior difference was reported; do not transfer sole-transporter status to humans.\nEvidence access: Primary abstract\nKnockout of the ergothioneine transporter ETT in zebrafish results in increased 8-oxoguanine levels. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25746775/ · DOI 10.1016/j.freeradbiomed.2015.02.026","model_system":"Retroviral insertion into transporter exon 1.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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