{"id":"a071e991-b873-5ae2-8091-9053b9cc7deb","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:opc-rapamycin","predicate":"decreases_in_recorded_experiment","statement":"Rapamycin blocked the SA-associated MBP increase.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"9aa9b280-584c-573f-8326-1d03933c06b9","mechanism_event_label":"Rapamycin blocked the SA-associated MBP increase.","subject":{"id":"9ed727d1-a681-51e2-ae44-34f734a47316","slug":"rapamycin","display_name":"Rapamycin / sirolimus","entity_type_key":"small_molecule"},"object":{"id":"f6d3d9d8-1cba-59d7-973a-b7decec87431","slug":"rat-mbp","display_name":"Rat myelin basic protein / Mbp","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"9aa9b280-584c-573f-8326-1d03933c06b9","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:opc-rapamycin-event","event_type":"experimental_observation","label":"Rapamycin blocked the SA-associated MBP increase.","description":"**Perturbations expose a conditional signaling route.** In those rat precursor cultures, rapamycin blocked the shikimic-acid-associated rise in MBP, while the MEK inhibitor U0126 did not abolish it. PI3K inhibitors wortmannin and LY294002 reduced the elevated mTOR-phosphorylation readout toward control. These are joint experimental contrasts supporting pathway dependence; they do not establish that shikimic acid binds mTOR or that all MEK activity is irrelevant. [Shikimic Acid Promotes Oligodendrocyte Precursor Cell Differentiation and Accelerates Remyelination in Mice.](https://pubmed.ncbi.nlm.nih.gov/30684125/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9ed727d1-a681-51e2-ae44-34f734a47316","slug":"rapamycin","display_name":"Rapamycin / sirolimus","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"rapamycin plus SA","sequence_order":0,"notes":""},{"entity":{"id":"f6d3d9d8-1cba-59d7-973a-b7decec87431","slug":"rat-mbp","display_name":"Rat myelin basic protein / Mbp","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"role":"joint exposure","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. 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Inhibitors do not identify the direct SA target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Rapamycin blocked the SA-associated MBP increase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Shikimic Acid Promotes Oligodendrocyte Precursor Cell Differentiation and Accelerates Remyelination in Mice. | 2019 | DOI 10.1007/s12264-018-0322-7 | PMID 30684125 | https://pubmed.ncbi.nlm.nih.gov/30684125/ | https://doi.org/10.1007/s12264-018-0322-7 | https://pmc.ncbi.nlm.nih.gov/articles/PMC6527532/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 71-71; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"19dc3282-51d9-5dd4-841e-d65b15772d3c","evidence_kind":"source_excerpt","locator":"Lines 71-71","start_line":71,"end_line":71,"excerpt":"**Perturbations expose a conditional signaling route.** In those rat precursor cultures, rapamycin blocked the shikimic-acid-associated rise in MBP, while the MEK inhibitor U0126 did not abolish it. PI3K inhibitors wortmannin and LY294002 reduced the elevated mTOR-phosphorylation readout toward control. These are joint experimental contrasts supporting pathway dependence; they do not establish that shikimic acid binds mTOR or that all MEK activity is irrelevant. [Shikimic Acid Promotes Oligodendrocyte Precursor Cell Differentiation and Accelerates Remyelination in Mice.](https://pubmed.ncbi.nlm.nih.gov/30684125/)","model_system":"Primary rat OPC joint exposure, 100 µg/mL SA.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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