{"id":"a0697e22-e4e3-5823-987c-3d52ee7b61dd","stable_key":"a9828b14-fbd7-57bf-9e01-0d52d1b42a1f:citrulline-citrin-shuttle","predicate":"overexpression_increased","statement":"Citrin overexpression increased malate-aspartate shuttle activity in transfected human cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"222e5387-c7be-5bfc-b867-c8d2affcf41d","mechanism_event_label":"The same transporter participates in moving reducing equivalents between compartments.","subject":{"id":"5252ba8d-8e58-5fa5-abdb-7bc97ef85818","slug":"slc25a13","display_name":"Human citrin / SLC25A13","entity_type_key":"protein"},"object":{"id":"eb50692a-525a-5d1a-b776-a3433046f2f0","slug":"malate-aspartate-shuttle-activity","display_name":"Malate-aspartate shuttle activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"222e5387-c7be-5bfc-b867-c8d2affcf41d","stable_key":"a9828b14-fbd7-57bf-9e01-0d52d1b42a1f:citrulline-citrin-shuttle-event","event_type":"biochemical_relationship","label":"The same transporter participates in moving reducing equivalents between compartments.","description":"Citrin overexpression increased malate-aspartate shuttle activity in transfected human cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"exchanged_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"exchanged_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5252ba8d-8e58-5fa5-abdb-7bc97ef85818","slug":"slc25a13","display_name":"Human citrin / SLC25A13","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eb50692a-525a-5d1a-b776-a3433046f2f0","slug":"malate-aspartate-shuttle-activity","display_name":"Malate-aspartate shuttle activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/citrulline-research/11566871.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"25151a91c1f832fb2e3f86421ef070fb3a7c92390b5b1e7c0038e1db1d6a0734\", \"start_char\": 0, \"end_char\": 1249, \"text_sha256\": \"25151a91c1f832fb2e3f86421ef070fb3a7c92390b5b1e7c0038e1db1d6a0734\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reconstituted transporter and transfected-cell assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Aspartate exchange for glutamate plus proton; external calcium stimulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell and liposome experiments; no evidence that calcium supplementation corrects citrin deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Citrulline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human citrin and aralar proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The same transporter participates in moving reducing equivalents between compartments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[citrulline-p11566871] Citrin and aralar1 are Ca(2+)-stimulated aspartate/glutamate transporters in mitochondria. (2001). https://pubmed.ncbi.nlm.nih.gov/11566871/ DOI: 10.1093/emboj/20.18.5060","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Inner mitochondrial membrane transport; malate-aspartate shuttle","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"64535eca-87a1-550c-a1bc-d766c3f36e57","evidence_kind":"source_excerpt","locator":"Lines 385-396","start_line":385,"end_line":396,"excerpt":"### citrulline-citrin-shuttle\nCitrin overexpression increased malate-aspartate shuttle activity in transfected human cells.\nCondition category: normal\nnutrient_topic: Citrulline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The same transporter participates in moving reducing equivalents between compartments.\norganism: Human citrin and aralar proteins\ntissue_or_cell_type: Inner mitochondrial membrane transport; malate-aspartate shuttle\nexperimental_model: Reconstituted transporter and transfected-cell assays\nlimitations: Cell and liposome experiments; no evidence that calcium supplementation corrects citrin deficiency.\nexposure: Aspartate exchange for glutamate plus proton; external calcium stimulation\nevidence_span: {\"source_cache\": \"artifacts/citrulline-research/11566871.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"25151a91c1f832fb2e3f86421ef070fb3a7c92390b5b1e7c0038e1db1d6a0734\", \"start_char\": 0, \"end_char\": 1249, \"text_sha256\": \"25151a91c1f832fb2e3f86421ef070fb3a7c92390b5b1e7c0038e1db1d6a0734\"}\n[citrulline-p11566871] Citrin and aralar1 are Ca(2+)-stimulated aspartate/glutamate transporters in mitochondria. 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